| Literature DB >> 27145383 |
Jingwei Huang1,2, Changyan Chen2, Hua Gu2, Chen Li2, Xing Fu3, Ming Jiang2,3, Hui Sun1,2,3, Jun Xu1, Jianmin Fang2,3, Lingjing Jin1.
Abstract
Mesencephalic astrocyte-derived neurotrophic factor (MANF) protects dopaminergic neurons from damage. In this study, we used MTT, immunohistochemistry, and TUNEL staining to investigate the protective effect of MANF in SH-SY5Y cells treated with 6-OHDA or overexpressed α-synuclein. Cleaved caspase-3 levels significantly increased in cells treated with 6-OHDA or overexpressed α-synuclein. 6-OHDA or α-synuclein overexpression that induced cleaved caspase-3 levels to increase was reduced by MANF treatment. In addition, MANF treatment upregulated GRP78 expressions in cells treated with 6-OHDA or overexpressed α-synuclein, and RNAi knockdown for GRP78 could block the MANF induced cell survival from 6-OHDA treatment. Furthermore, GRP78 overexpression inhibited 6-OHDA-induced apoptosis. Our data suggest that MANF inhibits apoptosis induced by 6-OHDA and overexpressed α-synuclein in SH-SY5Y cells via upregulating GRP78 in the transcriptional pattern.Entities:
Keywords: GRP78; MANF; Parkinson's disease; SH-SY5Y
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Year: 2016 PMID： 27145383 DOI： 10.1002/cbin.10621
Source DB: PubMed Journal: Cell Biol Int ISSN： 1065-6995 Impact factor: 3.612