Ü C Yazgan1, B Elbey2, S Kuş3, B Baykal4, I Keskin5, A Yılmaz6, A Şahin7. 1. Department of Physiology, Faculty of Medicine, Zirve University, Gaziantep, Turkey. 2. Departments of Immunology, Faculty of Medicine, Dicle University, Diyarbakır, 21280, Turkey. drbilalelbey@gmail.com. 3. Biochemistry, Faculty of Medicine, Dicle University, Diyarbakır, Turkey. 4. Medical Biology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey. 5. Anatomy, Faculty of Medicine, Dicle University, Diyarbakır, Turkey. 6. Family Medicine, Faculty of Medicine, Dicle University, Diyarbakır, Turkey. 7. Ophthalmology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.
Abstract
BACKGROUND: Methanol toxicity is one of the major public health problems because it can cause severe morbidity and mortality. Methanol intoxication causes changes in the balance between the production of free radicals and antioxidant capacity. AIMS: We aimed to investigate the effects of caffeic acid phenethyl ester (CAPE) on the total oxidant status, total antioxidant status (TAS), and oxidative stress index (OSI) parameters of the liver and the serum in a rat model of acute methanol intoxication. MATERIALS AND METHODS: Rats were treated with intraperitoneal (i.p.) Methotrexate (MTX) for 7 days. On the 8th day, i.p. Methanol was administered in the methanol, ethanol and CAPE groups. Four hours after methanol treatment, ethanol was injected i.p. in the ethanol group; CAPE (i.p.) in the CAPE group; serum physiologic i.p. in other groups. After 8 hours, rats were killed and the serum and the liver samples were obtained for biochemical analyses. RESULTS: The OSI value was significantly higher in the methanol group compared to the ethanol and CAPE groups. Serum TAS levels of the methanol group were significantly different compared to the control group, but not compared to the MTX group. The amelioration of oxidative stress was greater in the CAPE group compared to the ethanol group but was not statistically significant. CONCLUSION: This study demonstrates that CAPE treatment ameliorates oxidative stress in the serum and liver in a rat model of acute methanol intoxication.
BACKGROUND:Methanoltoxicity is one of the major public health problems because it can cause severe morbidity and mortality. Methanol intoxication causes changes in the balance between the production of free radicals and antioxidant capacity. AIMS: We aimed to investigate the effects of caffeic acid phenethyl ester (CAPE) on the total oxidant status, total antioxidant status (TAS), and oxidative stress index (OSI) parameters of the liver and the serum in a rat model of acute methanol intoxication. MATERIALS AND METHODS:Rats were treated with intraperitoneal (i.p.) Methotrexate (MTX) for 7 days. On the 8th day, i.p. Methanol was administered in the methanol, ethanol and CAPE groups. Four hours after methanol treatment, ethanol was injected i.p. in the ethanol group; CAPE (i.p.) in the CAPE group; serum physiologic i.p. in other groups. After 8 hours, rats were killed and the serum and the liver samples were obtained for biochemical analyses. RESULTS: The OSI value was significantly higher in the methanol group compared to the ethanol and CAPE groups. Serum TAS levels of the methanol group were significantly different compared to the control group, but not compared to the MTX group. The amelioration of oxidative stress was greater in the CAPE group compared to the ethanol group but was not statistically significant. CONCLUSION: This study demonstrates that CAPE treatment ameliorates oxidative stress in the serum and liver in a rat model of acute methanol intoxication.
Entities:
Keywords:
CAPE; Methanol intoxication; Oxidant stress index; Total antioxidant status; Total oxidant status
Authors: Esin Oğuzhanoğlu; Ahmet Cenk Andaç; Adnan Tüfek; Lütfi Yavuz; Hüseyin Vural; Osman Gökalp Journal: Turk J Med Sci Date: 2014 Impact factor: 0.973
Authors: Cebrail Gursul; Adalet Ozcicek; Mustafa Ozkaraca; Ali Sefa Mendil; Taha Abdulkadir Coban; Aynur Arslan; Fatih Ozcicek; Halis Suleyman Journal: Exp Anim Date: 2021-12-15