Jane E Libbey1, Daniel J Doty1, Jordan T Sim1, Matthew F Cusick2, June L Round1, Robert S Fujinami3. 1. Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA. 2. Division of Organ Transplantation, Northwestern University, Chicago, IL, USA. 3. Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA. Electronic address: Robert.Fujinami@hsc.utah.edu.
Abstract
OBJECTIVE: Many things can impact the reproducibility of results from laboratory to laboratory. For example, food from various sources can vary markedly in composition. We examined the effects of two different food sources, the Teklad Global Soy Protein-Free Extruded Rodent Diet (irradiated diet) and the Teklad Sterilizable Rodent Diet (autoclaved diet), on central nervous system disease. METHODS: Three preclinical models for human disease: Two different experimental autoimmune encephalomyelitis models (multiple sclerosis) and the Theiler's murine encephalomyelitis virus-induced seizure model (epilepsy), were examined for the effects of two different food sources on disease. RESULTS: We found that mice fed the irradiated diet had more severe clinical disease and enhanced seizures compared with animals provided the autoclaved diet in both experimental autoimmune encephalomyelitis models examined and in the Theiler's murine encephalomyelitis virus-induced seizure model, respectively. CONCLUSIONS: Therefore, just altering the source of food (lab chow) can have marked effects on disease severity and outcome.
OBJECTIVE: Many things can impact the reproducibility of results from laboratory to laboratory. For example, food from various sources can vary markedly in composition. We examined the effects of two different food sources, the Teklad Global Soy Protein-Free Extruded Rodent Diet (irradiated diet) and the Teklad Sterilizable Rodent Diet (autoclaved diet), on central nervous system disease. METHODS: Three preclinical models for human disease: Two different experimental autoimmune encephalomyelitis models (multiple sclerosis) and the Theiler's murine encephalomyelitis virus-induced seizure model (epilepsy), were examined for the effects of two different food sources on disease. RESULTS: We found that mice fed the irradiated diet had more severe clinical disease and enhanced seizures compared with animals provided the autoclaved diet in both experimental autoimmune encephalomyelitis models examined and in the Theiler's murine encephalomyelitis virus-induced seizure model, respectively. CONCLUSIONS: Therefore, just altering the source of food (lab chow) can have marked effects on disease severity and outcome.
Authors: John Michael S Sanchez; Ana Beatriz DePaula-Silva; Daniel J Doty; Amanda Truong; Jane E Libbey; Robert S Fujinami Journal: J Neurovirol Date: 2019-03-11 Impact factor: 2.643
Authors: J E Libbey; J M Sanchez; D J Doty; J T Sim; M F Cusick; J E Cox; K F Fischer; J L Round; R S Fujinami Journal: Benef Microbes Date: 2018-01-30 Impact factor: 4.205
Authors: John Michael S Sanchez; Ana Beatriz DePaula-Silva; Jane E Libbey; Robert S Fujinami Journal: Clin Immunol Date: 2020-03-07 Impact factor: 3.969