Mingming Zhang1, Yamin Lu2, Xin Liu3, Xiaobin Zhang4, Cuigai Zhang5, Wei Gao6, Yanqing Tie6. 1. Clinical laboratory, Hebei General Hospital, Shijiazhuang, China. zhangmm197612@126.com. 2. Department of nuclear medicine, Hebei General Hospital, Shijiazhuang, China. 3. Function Department, Hebei General Hospital, Shijiazhuang, China. 4. Clinical laboratory, Second Hospital of Hebei Medical University, Shijiazhuang, China. 5. Physical examination center, Hebei General Hospital, Shijiazhuang, China. 6. Clinical laboratory, Hebei General Hospital, Shijiazhuang, China.
Abstract
BACKGROUND: Relationship has been identified in sporadic reports between polymorphisms and hypercholesterolemia. However, the relationship between inflammatory cytokines and polymorphism of low-density lipoprotein receptor (LDL-R) gene in hypercholesterolemia is unclear. This study aimed to explore the relationship and significance between polymorphisms of LDL-R gene and serum Interleukin-2 (IL-2), IL-10 in patients with hypercholesterolemia. METHODS: PCR-RFLP and direct DNA sequencing assay were employed to determine polymorphism of LDL-R gene in 900 patients with hypercholesterolemia and 400 healthy cases. ELISA was applied to assay serum concentration of IL-2 and IL-10. Blood lipid indexes were tested in all cases. RESULTS: Compared with the healthy controls, level of IL-2 increased significantly, while IL-10 decreased significantly (P < 0.05). Correlation analysis showed that IL-2 was positively correlated with total cholesterol (TC), LDL-c, and genotype (r = 0.542, 0.410, 0.598, P < 0.05) and negatively correlated with HDL-c (r = -0.352, P < 0.05). Negative relationship also was found between TC, LDL-c, genotype, and IL-10 (r = -0.452, -0.390, -0.613, P < 0.05), and positive correlation between HDL-c and IL-10 (r = 0.398, P < 0.05). Multiple linear regression showed that genotypes and TC were independent factors affecting the levels of IL-2 and IL-10 (P < 0.05). CONCLUSION: IL-2 and IL-10 were related to gene polymorphisms of LDL-R, which might be involved in the development and progress of hypercholesterolemia.
BACKGROUND: Relationship has been identified in sporadic reports between polymorphisms and hypercholesterolemia. However, the relationship between inflammatory cytokines and polymorphism of low-density lipoprotein receptor (LDL-R) gene in hypercholesterolemia is unclear. This study aimed to explore the relationship and significance between polymorphisms of LDL-R gene and serum Interleukin-2 (IL-2), IL-10 in patients with hypercholesterolemia. METHODS: PCR-RFLP and direct DNA sequencing assay were employed to determine polymorphism of LDL-R gene in 900 patients with hypercholesterolemia and 400 healthy cases. ELISA was applied to assay serum concentration of IL-2 and IL-10. Blood lipid indexes were tested in all cases. RESULTS: Compared with the healthy controls, level of IL-2 increased significantly, while IL-10 decreased significantly (P < 0.05). Correlation analysis showed that IL-2 was positively correlated with total cholesterol (TC), LDL-c, and genotype (r = 0.542, 0.410, 0.598, P < 0.05) and negatively correlated with HDL-c (r = -0.352, P < 0.05). Negative relationship also was found between TC, LDL-c, genotype, and IL-10 (r = -0.452, -0.390, -0.613, P < 0.05), and positive correlation between HDL-c and IL-10 (r = 0.398, P < 0.05). Multiple linear regression showed that genotypes and TC were independent factors affecting the levels of IL-2 and IL-10 (P < 0.05). CONCLUSION:IL-2 and IL-10 were related to gene polymorphisms of LDL-R, which might be involved in the development and progress of hypercholesterolemia.
Authors: Daniel Kolbus; Maria Wigren; Irena Ljungcrantz; Ingrid Söderberg; Ragnar Alm; Harry Björkbacka; Jan Nilsson; Gunilla N Fredrikson Journal: Immunobiology Date: 2010-11-19 Impact factor: 3.144
Authors: Maik Drechsler; Remco T A Megens; Marc van Zandvoort; Christian Weber; Oliver Soehnlein Journal: Circulation Date: 2010-10-18 Impact factor: 29.690