Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Single-Plasmid-Based System for Efficient Noncanonical Amino Acid Mutagenesis in Cultured Mammalian Cells.

Literature DB >> 27120490

Single-Plasmid-Based System for Efficient Noncanonical Amino Acid Mutagenesis in Cultured Mammalian Cells.

Abstract

We describe a new expression system for efficient non-canonical amino acid mutagenesis in cultured mammalian cells by using the pyrrolysine tRNA synthetase/tRNACUA (Pyl) pair. A significant improvement in the incorporation of non-canonical amino acids into proteins was obtained by combining all the required genetic components into a single and compact vector that can be efficiently delivered to different mammalian cell lines by conventional transfection reagents.

Entities: Species

Keywords: amber suppression; genetic code expansion; protein engineering; protein expression; pyrrolysine

Mesh：

Substances：

Year: 2016 PMID： 27120490 DOI： 10.1002/cbic.201500681

Source DB: PubMed Journal: Chembiochem ISSN： 1439-4227 Impact factor: 3.164

Keyword Cloud
Cited

11 in total

1. Nonsense mutation-dependent reinitiation of translation in mammalian cells.

Authors: Sarit Cohen; Lior Kramarski; Shahar Levi; Noa Deshe; Oshrit Ben David; Eyal Arbely
Journal: Nucleic Acids Res Date: 2019-07-09 Impact factor: 16.971

Review 2. A molecular engineering toolbox for the structural biologist.

Authors: Galia T Debelouchina; Tom W Muir
Journal: Q Rev Biophys Date: 2017-01 Impact factor: 5.318

3. Broadening the Toolkit for Quantitatively Evaluating Noncanonical Amino Acid Incorporation in Yeast.

Authors: Jessica T Stieglitz; Kelly A Potts; James A Van Deventer
Journal: ACS Synth Biol Date: 2021-11-03 Impact factor: 5.110

4. Genetic code expansion in mammalian cells: A plasmid system comparison.

Authors: Wenyuan Zhou; Joshua S Wesalo; Jihe Liu; Alexander Deiters
Journal: Bioorg Med Chem Date: 2020-09-19 Impact factor: 3.641

5. Identification of permissive amber suppression sites for efficient non-canonical amino acid incorporation in mammalian cells.

Authors: Michael D Bartoschek; Enes Ugur; Tuan-Anh Nguyen; Geraldine Rodschinka; Michael Wierer; Kathrin Lang; Sebastian Bultmann
Journal: Nucleic Acids Res Date: 2021-06-21 Impact factor: 16.971

10. A straightforward approach for bioorthogonal labeling of proteins and organelles in live mammalian cells, using a short peptide tag.

Authors: Inbar Segal; Dikla Nachmias; Andres Konig; Ariel Alon; Eyal Arbely; Natalie Elia
Journal: BMC Biol Date: 2020-01-14 Impact factor: 7.431

Single-Plasmid-Based System for Efficient Noncanonical Amino Acid Mutagenesis in Cultured Mammalian Cells.

1. Nonsense mutation-dependent reinitiation of translation in mammalian cells.

Review 2. A molecular engineering toolbox for the structural biologist.

3. Broadening the Toolkit for Quantitatively Evaluating Noncanonical Amino Acid Incorporation in Yeast.

4. Genetic code expansion in mammalian cells: A plasmid system comparison.

5. Identification of permissive amber suppression sites for efficient non-canonical amino acid incorporation in mammalian cells.

6. Direct fluorescent-dye labeling of α-tubulin in mammalian cells for live cell and superresolution imaging.

Review 7. Recent Development of Genetic Code Expansion for Posttranslational Modification Studies.

8. Live Cell Imaging of Bioorthogonally Labelled Proteins Generated With a Single Pyrrolysine tRNA Gene.

Review 9. Using genetically incorporated unnatural amino acids to control protein functions in mammalian cells.

10. A straightforward approach for bioorthogonal labeling of proteins and organelles in live mammalian cells, using a short peptide tag.