Yong-Kang Liu1, Bao-Shan Hu2, Zhong-Liang Li2, Xu He3, Yong Li3, Li-Gong Lu4. 1. Department of Interventional Radiology, Cancer Center, Guangdong General Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, China. 2. Department of Interventional Radiology, Cancer Center, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, Guangdong, China. 3. Department of Interventional Radiology, Cancer Center, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, Guangdong, China. 4. Department of Interventional Radiology, Cancer Center, Guangdong General Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, China. lulg1234@126.com.
Abstract
PURPOSE: We adopted a new strategy to explore the relationship between the EMT process of CTCs and hepatocellular carcinoma (HCC). Furthermore, we intend to illustrate the potential diagnostic value of CTCs of distinct phenotypes in HCC. METHODS: The clinical data of 33 HCC patients and 10 healthy volunteers were collected retrospectively. By using the optimized CanPatrol CTC enrichment technique, patient blood samples of about 5 ml were collected, and CTCs were identified and characterized. The first step of this detection process was to isolate CTCs via a filter-based method; then, an RNA in situ hybridization (RNA-ISH) technique based on the branched DNA signal amplification technology was used to classify the CTCs according to EMT markers. The relationships between HCC CTCs and clinical characteristics were analyzed. RESULTS: The number of epithelial CTCs was related to tumor size (r = 0.456, p = 0.008), epithelial-mesenchymal-mixed CTCs were related to tumor number (r = 0.421, p = 0.015), and mesenchymal CTC was associated with metastasis (r = 0.375, p = 0.032). There was no significant correlation between CTC number and other clinicopathological factors, such as age, serum AFP level or cirrhosis. CONCLUSIONS: Epithelial-mesenchymal-mixed CTCs seem to play an important role in EMT transition in HCC, mixed CTCs might be a vital factor for intrahepatic metastasis, and mesenchymal CTCs had the potential to be a predictor of extrahepatic metastasis.
PURPOSE: We adopted a new strategy to explore the relationship between the EMT process of CTCs and hepatocellular carcinoma (HCC). Furthermore, we intend to illustrate the potential diagnostic value of CTCs of distinct phenotypes in HCC. METHODS: The clinical data of 33 HCC patients and 10 healthy volunteers were collected retrospectively. By using the optimized CanPatrol CTC enrichment technique, patient blood samples of about 5 ml were collected, and CTCs were identified and characterized. The first step of this detection process was to isolate CTCs via a filter-based method; then, an RNA in situ hybridization (RNA-ISH) technique based on the branched DNA signal amplification technology was used to classify the CTCs according to EMT markers. The relationships between HCC CTCs and clinical characteristics were analyzed. RESULTS: The number of epithelial CTCs was related to tumor size (r = 0.456, p = 0.008), epithelial-mesenchymal-mixed CTCs were related to tumor number (r = 0.421, p = 0.015), and mesenchymal CTC was associated with metastasis (r = 0.375, p = 0.032). There was no significant correlation between CTC number and other clinicopathological factors, such as age, serum AFP level or cirrhosis. CONCLUSIONS: Epithelial-mesenchymal-mixed CTCs seem to play an important role in EMT transition in HCC, mixed CTCs might be a vital factor for intrahepatic metastasis, and mesenchymal CTCs had the potential to be a predictor of extrahepatic metastasis.
Authors: Walid A Osta; Yian Chen; Kaidi Mikhitarian; Michael Mitas; Mohamed Salem; Yusuf A Hannun; David J Cole; William E Gillanders Journal: Cancer Res Date: 2004-08-15 Impact factor: 12.701
Authors: Tobias M Gorges; Ingeborg Tinhofer; Michael Drosch; Lars Röse; Thomas M Zollner; Thomas Krahn; Oliver von Ahsen Journal: BMC Cancer Date: 2012-05-16 Impact factor: 4.430
Authors: Mario Giuliano; Antonio Giordano; Summer Jackson; Kenneth R Hess; Ugo De Giorgi; Michal Mego; Beverly C Handy; Naoto T Ueno; Ricardo H Alvarez; Michelino De Laurentiis; Sabino De Placido; Vicente Valero; Gabriel N Hortobagyi; James M Reuben; Massimo Cristofanilli Journal: Breast Cancer Res Date: 2011-06-15 Impact factor: 6.466
Authors: Glenn Deng; Michael Herrler; David Burgess; Edward Manna; David Krag; Julian F Burke Journal: Breast Cancer Res Date: 2008-08-07 Impact factor: 6.466