| Literature DB >> 27113041 |
Jing Zhou1, Chencheng Wang1, Kunshan Zhang2, Yingying Wang2, Xi Gong1, Yanlu Wang1, Siguang Li3, Yuping Luo4,5.
Abstract
Lineage specific human embryonic stem cell (hESC) reporter cell line is a versatile tool for biological studies on real time monitoring of differentiation, physiological and biochemical features of special cell types and pathological mechanism of disease. Here we report the generation of ChAT-zsGreen reporter hESC line that express zsGreen under the control of the choline acetyltransferase (ChAT) promoter using CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats)/Cas9 system. We show that the ChAT-zsGreen hESC reporter cell lines retain the features of undifferentiated hESC. After cholinergic neuronal differentiation, cholinergic neurons were clearly labeled with green fluorescence protein (zsGreen). The ChAT-zsGreen reporter hESC lines are invaluable not only for the monitoring cholinergic neuronal differentiation but also for study physiological and biochemical hallmarks of cholinergic neurons.Entities:
Keywords: CRISPR/Cas9 system; Cholinergic neurons; Embryonic stem cell; Reporter cell line; zsGreen
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Year: 2016 PMID： 27113041 DOI： 10.1007/s11064-016-1918-9
Source DB: PubMed Journal: Neurochem Res ISSN： 0364-3190 Impact factor: 3.996