Massimo Marrelli1,2, Antonella Pujia1, Francesca Palmieri1, Roberto Gatto3, Giovanni Falisi3, Marco Gargari4, Silvia Caruso3, Davide Apicella2, Claudio Rastelli3, Gianna Maria Nardi5, Francesco Paduano1, Marco Tatullo6,2. 1. Tecnologica Research Institute, Biomedical Section, Crotone, Italy. 2. Calabrodental clinic, Biomaterials unit, Crotone, Italy. 3. Department of Life, Health and Environmental Sciences, School of Dentistry, University of L'Aquila, L'Aquila, Italy. 4. Department of Clinical Sciences and Translational Medicine, University of Rome "Tor Vergata", Rome, Italy. 5. Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, Rome, Italy. 6. Tecnologica Research Institute, Biomedical Section, Crotone, Italy marco.tatullo@tecnologicasrl.com.
Abstract
INTRODUCTION: Studies on biomaterials involve assays aimed to assess the interactions between the biomaterial and the cells seeded on its surface. However, the morphology of biomaterials is heterogeneous and it could be tricky to standardize the results among different biomaterials and the classic plastic plates. In this light, we decided to create, by means of computer-aided design (CAD) technology, a standardized sample model, with equal shape and sizes, able to fit into a classic shape of a 96-wells tissue culture plate (TCP). METHODS: The design of this sample consists of a hole in the top in order to allow the injected cells to settle without them being able to slip from the sides of the sample to the bottom of the TCP wells. This CAD project is made using the software Pro-Engineer. The sample will totally fill the wells of the 96-well TCP. Dental pulp stem cells have been used to assess the ability of the different sample to support and promote the cell proliferation. RESULTS: Twelve titanium, 12 gold-palladium, and 12 zirconium oxide customized samples were designed by means of the software cam powermill, by importing the .stl file created in Pro-Engineer software. The proliferation rate of the tested scaffolds showed to be similar to the control in the group with the customized shape. CONCLUSION: We think that our method can be useful to test different types of scaffolds when a greater accuracy of the measurements is desirable in order to verify the cell behavior of these scaffolds. Our innovative method can improve the standardization process in the evaluation of cell behavior on different biomaterials to open the way to more reliable tests on biomatrices functionalized with drugs or growth factors applied to the future regenerative medicine.
INTRODUCTION: Studies on biomaterials involve assays aimed to assess the interactions between the biomaterial and the cells seeded on its surface. However, the morphology of biomaterials is heterogeneous and it could be tricky to standardize the results among different biomaterials and the classic plastic plates. In this light, we decided to create, by means of computer-aided design (CAD) technology, a standardized sample model, with equal shape and sizes, able to fit into a classic shape of a 96-wells tissue culture plate (TCP). METHODS: The design of this sample consists of a hole in the top in order to allow the injected cells to settle without them being able to slip from the sides of the sample to the bottom of the TCP wells. This CAD project is made using the software Pro-Engineer. The sample will totally fill the wells of the 96-well TCP. Dental pulp stem cells have been used to assess the ability of the different sample to support and promote the cell proliferation. RESULTS: Twelve titanium, 12 gold-palladium, and 12 zirconium oxide customized samples were designed by means of the software cam powermill, by importing the .stl file created in Pro-Engineer software. The proliferation rate of the tested scaffolds showed to be similar to the control in the group with the customized shape. CONCLUSION: We think that our method can be useful to test different types of scaffolds when a greater accuracy of the measurements is desirable in order to verify the cell behavior of these scaffolds. Our innovative method can improve the standardization process in the evaluation of cell behavior on different biomaterials to open the way to more reliable tests on biomatrices functionalized with drugs or growth factors applied to the future regenerative medicine.
Authors: Ferry P W Melchels; Ana M C Barradas; Clemens A van Blitterswijk; Jan de Boer; Jan Feijen; Dirk W Grijpma Journal: Acta Biomater Date: 2010-06-16 Impact factor: 8.947
Authors: Marco Tatullo; Massimo Marrelli; Giovanni Falisi; Claudio Rastelli; Francesca Palmieri; Marco Gargari; Barbara Zavan; Francesco Paduano; Vincenzo Benagiano Journal: Int J Immunopathol Pharmacol Date: 2015-11-26 Impact factor: 3.219
Authors: A Ballini; F Mastrangelo; G Gastaldi; L Tettamanti; N Bukvic; S Cantore; T Cocco; R Saini; A Desiate; E Gherlone; S Scacco Journal: J Biol Regul Homeost Agents Date: 2015 Oct-Dec Impact factor: 1.711
Authors: Ferry P W Melchels; Beatrice Tonnarelli; Andy L Olivares; Ivan Martin; Damien Lacroix; Jan Feijen; David J Wendt; Dirk W Grijpma Journal: Biomaterials Date: 2011-02-01 Impact factor: 12.479