Literature DB >> 27103115

Native MS and ECD Characterization of a Fab-Antigen Complex May Facilitate Crystallization for X-ray Diffraction.

Ying Zhang1,2, Weidong Cui1, Aaron T Wecksler3, Hao Zhang1, Patricia Molina3, Galahad Deperalta3, Michael L Gross4.   

Abstract

Native mass spectrometry (MS) and top-down electron-capture dissociation (ECD) combine as a powerful approach for characterizing large proteins and protein assemblies. Here, we report their use to study an antibody Fab (Fab-1)-VEGF complex in its near-native state. Native ESI with analysis by FTICR mass spectrometry confirms that VEGF is a dimer in solution and that its complex with Fab-1 has a binding stoichiometry of 2:2. Applying combinations of collisionally activated dissociation (CAD), ECD, and infrared multiphoton dissociation (IRMPD) allows identification of flexible regions of the complex, potentially serving as a guide for crystallization and X-ray diffraction analysis. Graphical Abstract ᅟ.

Entities:  

Keywords:  Electron capture dissociation (ECD); Fab-Antigen Complex; Fourier transform ICR mass spectrometry; High-order structure (HOS); Native MS; VEGF; X-ray crystallography

Mesh：

Substances：

Year:  2016        PMID： 27103115      PMCID： PMC4899112          DOI： 10.1007/s13361-016-1398-9

Source DB:  PubMed          Journal:  J Am Soc Mass Spectrom        ISSN： 1044-0305            Impact factor:   3.109


  36 in total

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Authors:  Zhongqi Zhang; Hai Pan; Xiaoyu Chen
Journal:  Mass Spectrom Rev       Date:  2009 Jan-Feb       Impact factor: 10.946

Review 2.  Studying noncovalent protein complexes by electrospray ionization mass spectrometry.

Authors:  J A Loo
Journal:  Mass Spectrom Rev       Date:  1997 Jan-Feb       Impact factor: 10.946

3.  Electron transfer dissociation provides higher-order structural information of native and partially unfolded protein complexes.

Authors:  Frederik Lermyte; Frank Sobott
Journal:  Proteomics       Date:  2015-07-22       Impact factor: 3.984

4.  Identifying disordered regions in proteins by limited proteolysis.

Authors:  Angelo Fontana; Patrizia Polverino de Laureto; Barbara Spolaore; Erica Frare
Journal:  Methods Mol Biol       Date:  2012

5.  Pulsed hydrogen-deuterium exchange mass spectrometry probes conformational changes in amyloid beta (Aβ) peptide aggregation.

Authors:  Ying Zhang; Don L Rempel; Jun Zhang; Anuj K Sharma; Liviu M Mirica; Michael L Gross
Journal:  Proc Natl Acad Sci U S A       Date:  2013-08-19       Impact factor: 11.205

6.  Conformational analysis of recombinant monoclonal antibodies with hydrogen/deuterium exchange mass spectrometry.

Authors:  Damian Houde; John R Engen
Journal:  Methods Mol Biol       Date:  2013

7.  Native top-down mass spectrometry for the structural characterization of human hemoglobin.

Authors:  Jiang Zhang; G Reza Malmirchegini; Robert T Clubb T Clubb; Joseph A Loo
Journal:  Eur J Mass Spectrom (Chichester)       Date:  2015       Impact factor: 1.067

Review 8.  Angiogenesis in cancer, vascular, rheumatoid and other disease.

Authors:  J Folkman
Journal:  Nat Med       Date:  1995-01       Impact factor: 53.440

9.  Revealing ligand binding sites and quantifying subunit variants of noncovalent protein complexes in a single native top-down FTICR MS experiment.

Authors:  Huilin Li; Piriya Wongkongkathep; Steve L Van Orden; Rachel R Ogorzalek Loo; Joseph A Loo
Journal:  J Am Soc Mass Spectrom       Date:  2014-06-10       Impact factor: 3.109

10.  In situ proteolysis to generate crystals for structure determination: an update.

Authors:  Amy Wernimont; Aled Edwards
Journal:  PLoS One       Date:  2009-04-07       Impact factor: 3.240
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  12 in total

Review 1.  Potent Neutralization of Staphylococcal Enterotoxin B In Vivo by Antibodies that Block Binding to the T-Cell Receptor.

Authors:  Gang Chen; Hatice Karauzum; Hua Long; Danielle Carranza; Frederick W Holtsberg; Katie A Howell; Laura Abaandou; Bojie Zhang; Nick Jarvik; Wei Ye; Grant C Liao; Michael L Gross; Daisy W Leung; Gaya K Amarasinghe; M Javad Aman; Sachdev S Sidhu
Journal:  J Mol Biol       Date:  2019-03-27       Impact factor: 5.469

Review 2.  Radical solutions: Principles and application of electron-based dissociation in mass spectrometry-based analysis of protein structure.

Authors:  Frederik Lermyte; Dirk Valkenborg; Joseph A Loo; Frank Sobott
Journal:  Mass Spectrom Rev       Date:  2018-02-09       Impact factor: 10.946

3.  Epitope characterization of anti-JAM-A antibodies using orthogonal mass spectrometry and surface plasmon resonance approaches.

Authors:  Guillaume Terral; Thierry Champion; François Debaene; Olivier Colas; Maxime Bourguet; Elsa Wagner-Rousset; Nathalie Corvaia; Alain Beck; Sarah Cianferani
Journal:  MAbs       Date:  2017-09-21       Impact factor: 5.857

4.  Mapping the Binding Interface of VEGF and a Monoclonal Antibody Fab-1 Fragment with Fast Photochemical Oxidation of Proteins (FPOP) and Mass Spectrometry.

Authors:  Ying Zhang; Aaron T Wecksler; Patricia Molina; Galahad Deperalta; Michael L Gross
Journal:  J Am Soc Mass Spectrom       Date:  2017-03-02       Impact factor: 3.109

Review 5.  A PERSPECTIVE ON PERSONAL CONTRIBUTIONS TO FT-ICR MASS SPECTROMETRY.

Authors:  Michael L Gross; Don L Rempel
Journal:  Mass Spectrom Rev       Date:  2020-09-22       Impact factor: 10.946

6.  Native Mass Spectrometry at the Convergence of Structural Biology and Compositional Proteomics.

Authors:  Kevin Jooß; John P McGee; Neil L Kelleher
Journal:  Acc Chem Res       Date:  2022-06-24       Impact factor: 24.466

Review 7.  High-Resolution Native Mass Spectrometry.

Authors:  Sem Tamara; Maurits A den Boer; Albert J R Heck
Journal:  Chem Rev       Date:  2021-08-20       Impact factor: 72.087

8.  Hydrogen-deuterium exchange mass spectrometry identifies spatially distinct antibody epitopes on domain III of the Zika virus envelope protein.

Authors:  Jagat Adhikari; Haiyan Zhao; Estefania Fernandez; Yining Huang; Michael S Diamond; Daved H Fremont; Michael L Gross
Journal:  J Mass Spectrom       Date:  2020-11-16       Impact factor: 2.394

9.  Mapping a Conformational Epitope of Hemagglutinin A Using Native Mass Spectrometry and Ultraviolet Photodissociation.

Authors:  M Rachel Mehaffey; Jiwon Lee; Jiwon Jung; Michael B Lanzillotti; Edwin E Escobar; Keith R Morgenstern; George Georgiou; Jennifer S Brodbelt
Journal:  Anal Chem       Date:  2020-08-12       Impact factor: 6.986

10.  Native Mass Spectrometry Characterizes the Photosynthetic Reaction Center Complex from the Purple Bacterium Rhodobacter sphaeroides.

Authors:  Hao Zhang; Lucas B Harrington; Yue Lu; Mindy Prado; Rafael Saer; Don Rempel; Robert E Blankenship; Michael L Gross
Journal:  J Am Soc Mass Spectrom       Date:  2016-08-09       Impact factor: 3.109
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