Sandro Heuke1,2, Olga Chernavskaia1,2, Thomas Bocklitz2, Fisseha Bekele Legesse1,2, Tobias Meyer1,2, Denis Akimov1, Olaf Dirsch3, Günther Ernst1,4, Ferdinand von Eggeling1,2,4, Iver Petersen5, Orlando Guntinas-Lichius4, Michael Schmitt2, Jürgen Popp6,7. 1. Leibniz Institute of Photonic Technology, Jena, Germany. 2. Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University, Jena, Germany. 3. Institute of Pathology, Klinikum Chemnitz, Chemnitz, Germany. 4. Department of Otorhinolaryngology, Jena University Hospital, Jena, Germany. 5. Institute of Pathology, Jena University Hospital, Jena, Germany. 6. Leibniz Institute of Photonic Technology, Jena, Germany. Juergen.Popp@ipht-jena.de. 7. Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University, Jena, Germany. Juergen.Popp@ipht-jena.de.
Abstract
BACKGROUND: Treatment of early cancer stages is deeply connected to a good prognosis, a moderate reduction of the quality of life, and comparably low treatment costs. METHODS: Head and neck squamous cell carcinomas were investigated using the multimodal combination of coherent anti-Stokes Raman scattering (CARS), two-photon excited fluorescence (TPEF), and second-harmonic generation (SHG) microscopy. RESULTS: An increased median TPEF to CARS contrast was found comparing cancerous and healthy squamous epithelium with a p value of 1.8·10(-10) . A following comprehensive image analysis was able to predict the diagnosis of imaged tissue sections with an overall accuracy of 90% for a 4-class model. CONCLUSION: Nonlinear multimodal imaging is verified objectively as a valuable diagnostic tool that complements conventional staining protocols and can serve as filter in future clinical routine reducing the pathologist's workload.
BACKGROUND: Treatment of early cancer stages is deeply connected to a good prognosis, a moderate reduction of the quality of life, and comparably low treatment costs. METHODS: Head and neck squamous cell carcinomas were investigated using the multimodal combination of coherent anti-Stokes Raman scattering (CARS), two-photon excited fluorescence (TPEF), and second-harmonic generation (SHG) microscopy. RESULTS: An increased median TPEF to CARS contrast was found comparing cancerous and healthy squamous epithelium with a p value of 1.8·10(-10) . A following comprehensive image analysis was able to predict the diagnosis of imaged tissue sections with an overall accuracy of 90% for a 4-class model. CONCLUSION: Nonlinear multimodal imaging is verified objectively as a valuable diagnostic tool that complements conventional staining protocols and can serve as filter in future clinical routine reducing the pathologist's workload.
Authors: Sandro Heuke; Kevin Unger; Samira Khadir; Kamal Belkebir; Patrick C Chaumet; Hervé Rigneault; Anne Sentenac Journal: Opt Express Date: 2019-08-05 Impact factor: 3.894
Authors: Elen Tolstik; Nairveen Ali; Shuxia Guo; Paul Ebersbach; Dorothe Möllmann; Paula Arias-Loza; Johann Dierks; Irina Schuler; Erik Freier; Jörg Debus; Hideo A Baba; Peter Nordbeck; Thomas Bocklitz; Kristina Lorenz Journal: Int J Mol Sci Date: 2022-05-11 Impact factor: 6.208
Authors: Olga Chernavskaia; Sandro Heuke; Michael Vieth; Oliver Friedrich; Sebastian Schürmann; Raja Atreya; Andreas Stallmach; Markus F Neurath; Maximilian Waldner; Iver Petersen; Michael Schmitt; Thomas Bocklitz; Jürgen Popp Journal: Sci Rep Date: 2016-07-13 Impact factor: 4.379
Authors: Elena Beletkaia; Behdad Dashtbozorg; Rubin G Jansen; Theo J M Ruers; Herman L Offerhaus Journal: J Biomed Opt Date: 2020-09 Impact factor: 3.170