D Huang1, F Fang, F Xu. 1. Department of Pediatric Intensive Care Unit, Guizhou Provincial People's Hospital, GuiYang, China. xu_fengmail@163.com.
Abstract
OBJECTIVE: It has been reported that inflammation of lung could be induced by proinflammatory factor under hyperoxia, which may be attributed by increasing generation of reactive oxygen species (ROS). MATERIALS AND METHODS: In the present study, with human epithelial lung cancer cell line A549 treated with hyperoxia as in vitro model, we found that hyperoxia stimulation induced TLR2/4 activity in A549 cells. ROS inhibitor NAC was used to investigate the role of ROS in hyperoxia-induced inflammatory cytokines secretion. RESULTS: Results of mRNA to protein level showed that elevated TLR2/4 activity and hyperoxia-induced inflammatory cytokines secretion could be significantly attenuated by NAC. EMSA results showed the activation of nuclear factor-κB (NF-κB) increased after 2-h hyperoxia stimulation, and the ROS inhibitor blocked TLR2/4 and NF-κB activity. CONCLUSIONS: Data suggested that the TLR2/4-NF-κB pathway is involved in hyperoxia-induced inflammatory cytokines secretion in A549 human type II alveolar epithelial cells.
OBJECTIVE: It has been reported that inflammation of lung could be induced by proinflammatory factor under hyperoxia, which may be attributed by increasing generation of reactive oxygen species (ROS). MATERIALS AND METHODS: In the present study, with humanepithelial lung cancer cell line A549 treated with hyperoxia as in vitro model, we found that hyperoxia stimulation induced TLR2/4 activity in A549 cells. ROS inhibitor NAC was used to investigate the role of ROS in hyperoxia-induced inflammatory cytokines secretion. RESULTS: Results of mRNA to protein level showed that elevated TLR2/4 activity and hyperoxia-induced inflammatory cytokines secretion could be significantly attenuated by NAC. EMSA results showed the activation of nuclear factor-κB (NF-κB) increased after 2-h hyperoxia stimulation, and the ROS inhibitor blocked TLR2/4 and NF-κB activity. CONCLUSIONS: Data suggested that the TLR2/4-NF-κB pathway is involved in hyperoxia-induced inflammatory cytokines secretion in A549 human type II alveolar epithelial cells.
Authors: Elizabeth G Damato; Seth J Fillioe; Seunghee P Margevicius; Ryan S Mayes; Jonathan E Somogyi; Ian S Vannix; Alireza Abdollahifar; Anthony M Turner; Lidia S Ilcus; Michael J Decker Journal: Front Physiol Date: 2022-04-28 Impact factor: 4.755
Authors: Ana Belén Herrera-Campos; Esteban Zamudio-Martinez; Daniel Delgado-Bellido; Mónica Fernández-Cortés; Luis M Montuenga; F Javier Oliver; Angel Garcia-Diaz Journal: Cancers (Basel) Date: 2022-05-31 Impact factor: 6.575
Authors: Ricardo Alva; Maha Mirza; Adam Baiton; Lucas Lazuran; Lyuda Samokysh; Ava Bobinski; Cale Cowan; Alvin Jaimon; Dede Obioru; Tala Al Makhoul; Jeffrey A Stuart Journal: Cell Biol Toxicol Date: 2022-09-16 Impact factor: 6.819