Literature DB >> 27087788

Enhancing the Linear Dynamic Range in Multi-Channel Single Photon Detector beyond 7OD.

Dmytro Gudkov1, George Gudkov2, Boris Gorbovitski1, Vera Gorfinkel2.   

Abstract

We present design, implementation, and characterization of a single photon detector based on 32-channel PMT sensor [model H7260-20, Hamamatsu]. The developed high speed electronics enables the photon counting with linear dynamic range (LDR) up to 108count/s per detector's channel. The experimental characterization and Monte-Carlo simulations showed that in the single photon counting mode the LDR of the PMT sensor is limited by (i) "photon" pulse width (current pulse) of 900ps and (ii) substantial decrease of amplitudes of current pulses for count rates exceeding 108 count/s. The multi-channel architecture of the detector and the developed firm/software allow further expansion of the dynamic range of the device by 32-fold by using appropriate beam shaping. The developed single photon counting detector was tested for the detection of fluorescence labeled microbeads in capillary flow.

Entities:  

Keywords:  fluorescence detection; photomultipliers; single photon counting

Year:  2015        PMID: 27087788      PMCID: PMC4827332          DOI: 10.1109/JSEN.2015.2471277

Source DB:  PubMed          Journal:  IEEE Sens J        ISSN: 1530-437X            Impact factor:   3.301


  7 in total

1.  Dynamic range of fluorescence detection and base-calling accuracy in DNA sequencer based on single-photon counting.

Authors:  Dmitri N Gavrilov; Boris Gorbovitski; Mikhail Gouzman; Georgiy Gudkov; Andrei Stepoukhovitch; Victor Ruskovoloshin; Andrei Tsuprik; Georgiy Tyshko; Olga Bilenko; Olga Kosobokova; Serge Luryi; Vera Gorfinkel
Journal:  Electrophoresis       Date:  2003-04       Impact factor: 3.535

2.  Encoding microcarriers by spatial selective photobleaching.

Authors:  Kevin Braeckmans; Stefaan C De Smedt; Chris Roelant; Marc Leblans; Rudi Pauwels; Joseph Demeester
Journal:  Nat Mater       Date:  2003-03       Impact factor: 43.841

3.  Ultra sensitive sensor with enhanced dynamic range for high speed detection of multi-color fluorescence radiation.

Authors:  A Tsupryk; I Tovkach; D Gavrilov; O Kosobokova; G Gudkov; G Tyshko; B Gorbovitski; V Gorfinkel
Journal:  Biosens Bioelectron       Date:  2008-01-19       Impact factor: 10.618

Review 4.  Critical review of recent developments in fluorescence detection for capillary electrophoresis.

Authors:  C E MacTaylor; A G Ewing
Journal:  Electrophoresis       Date:  1997-11       Impact factor: 3.535

5.  Detection of multi-color fluorescent objects with single photon spectrometer.

Authors:  Dmytro Gudkov; Dmitri Gavrilov; A Tsupryk; Ivan Tovkach; George Gudkov; Olga Kosobokova; Marina Cherevishnik; Boris Gorbovitski; Vera Gorfinkel
Journal:  Biosens Bioelectron       Date:  2012-07-20       Impact factor: 10.618

6.  Statistical treatment of photon/electron counting: extending the linear dynamic range from the dark count rate to saturation.

Authors:  David J Kissick; Ryan D Muir; Garth J Simpson
Journal:  Anal Chem       Date:  2010-11-29       Impact factor: 6.986

7.  Spectroscopic tags using dye-embedded nanoparticles and surface-enhanced Raman scattering.

Authors:  William E Doering; Shuming Nie
Journal:  Anal Chem       Date:  2003-11-15       Impact factor: 6.986

  7 in total

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