Justin R Ryder1, Michael J O'Connell2, Kyle D Rudser2, Claudia K Fox1, Anna N Solovey3,4, Robert P Hebbel3,4, Aaron S Kelly1,4. 1. Department of Pediatrics, University of Minnesota Medical School, Minneapolis, MN 55455, USA. 2. Division of Biostatistics, School of Public Health, Minneapolis, MN 55455, USA. 3. Vascular Biology Center, Division of Hematology, Oncology & Transplantation, University of Minnesota Medical School, Minneapolis, MN 55455, USA. 4. Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
Abstract
INTRODUCTION: We examined the reproducibility of circulating endothelial cells (CEC) enumeration and activation among youth. MATERIALS AND METHODS: CECs from 151 youth were measured at baseline and 1 week follow-up. Enumeration of CEC in fresh whole blood was determined by direct assessment of buffy coat smears (CD146+ nucleated cells) and activated CEC (%VCAM-1 expression) was determined after immunomagnetic enrichment and co-staining of nuclei, plus positivity for P1H12 and VCAM-1. RESULTS: No statistically significant difference in CEC enumeration (1.2 ± 2.5 vs 1.3 ± 2.2 CEC/milliliter of whole blood, p = 0.745) or activated CEC (57.1 ± 24.4 vs 58.0 ± 21.3 %VCAM-1, p = 0.592) between baseline and 1 week follow-up. CONCLUSION: On a cohort basis, CEC enumeration and activation are reproducible in youth. Relatively high individual biological variability may limit its clinical utility.
INTRODUCTION: We examined the reproducibility of circulating endothelial cells (CEC) enumeration and activation among youth. MATERIALS AND METHODS: CECs from 151 youth were measured at baseline and 1 week follow-up. Enumeration of CEC in fresh whole blood was determined by direct assessment of buffy coat smears (CD146+ nucleated cells) and activated CEC (%VCAM-1 expression) was determined after immunomagnetic enrichment and co-staining of nuclei, plus positivity for P1H12 and VCAM-1. RESULTS: No statistically significant difference in CEC enumeration (1.2 ± 2.5 vs 1.3 ± 2.2 CEC/milliliter of whole blood, p = 0.745) or activated CEC (57.1 ± 24.4 vs 58.0 ± 21.3 %VCAM-1, p = 0.592) between baseline and 1 week follow-up. CONCLUSION: On a cohort basis, CEC enumeration and activation are reproducible in youth. Relatively high individual biological variability may limit its clinical utility.
Authors: Andrew D Blann; Alexander Woywodt; Francesco Bertolini; Todd M Bull; Jill P Buyon; Robert M Clancy; Marion Haubitz; Robert P Hebbel; Gregory Y H Lip; Patrizia Mancuso; Jose Sampol; Anna Solovey; Françoise Dignat-George Journal: Thromb Haemost Date: 2005-02 Impact factor: 5.249
Authors: Aaron S Kelly; Robert P Hebbel; Anna N Solovey; Sarah Jane Schwarzenberg; Andrea M Metzig; Antoinette Moran; Alan R Sinaiko; David R Jacobs; Julia Steinberger Journal: J Pediatr Date: 2010-06-14 Impact factor: 4.406
Authors: Todd M Bull; Heiko Golpon; Robert P Hebbel; Anna Solovey; Carlyne D Cool; Rubin M Tuder; Mark W Geraci; Norbert F Voelkel Journal: Thromb Haemost Date: 2003-10 Impact factor: 5.249
Authors: D C Robbins; L Andersen; R Bowsher; R Chance; B Dinesen; B Frank; R Gingerich; D Goldstein; H M Widemeyer; S Haffner; C N Hales; L Jarett; K Polonsky; D Porte; J Skyler; G Webb; K Gallagher Journal: Diabetes Date: 1996-02 Impact factor: 9.461
Authors: Marilyne Levy; Damien Bonnet; Laetitia Mauge; David S Celermajer; Pascale Gaussem; David M Smadja Journal: PLoS One Date: 2013-06-10 Impact factor: 3.240
Authors: Elise F Northrop; Liming C Milbauer; Kyle D Rudser; Claudia K Fox; Anna N Solovey; Alexander M Kaizer; Robert P Hebbel; Aaron S Kelly; Justin R Ryder Journal: Biomark Med Date: 2019-11-15 Impact factor: 2.851
Authors: Katelyn E Uithoven; Justin R Ryder; Roland Brown; Kyle D Rudser; Nicholas G Evanoff; Donald R Dengel; Aaron S Kelly Journal: J Vasc Diagn Interv Date: 2017-01-13
Authors: Erica G Soltero; Anna N Solovey; Robert P Hebbel; Elise F Palzer; Justin R Ryder; Gabriel Q Shaibi; Micah Olson; Claudia K Fox; Kyle D Rudser; Donald R Dengel; Nicholas G Evanoff; Aaron S Kelly Journal: J Am Heart Assoc Date: 2020-12-29 Impact factor: 6.106