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Literature DB >> 270681

Sequence relationship between long and short repetitive DNA of the rat: a preliminary report.

J R Wu, W R Pearson, J W Posakony, J Bonner.

Abstract

Long and short repetitive sequences of rat DNA can be isolated and characterized. Long [greater than 1.5 kilobases (kb)] sequences can be separated from short (0.2-0.4 kb) sequences by exclusion chromatography after renaturation of 4-kb DNA fragments to a repetitive Cot and digestion with the single-strand-specific S1 nuclease. (Cot is the initial concentration of DNA in mol of nucleotides/liter multiplied by time in sec.) Long repetitive DNA can be driven by an excess of whole rat DNA can also be used to drive tracer quantities of either long (self-renaturation) or short repetitive DNA. Both the extent and the rate of the renaturations are found to be similar, suggesting that long and short DNA fragments share sequences. When long repetitive DNA is used to drive whole DNA tracers of various lengths, a 3.2-kb interspersion period is found. These data are consistent with the concept that short repetitive sequences are present within long repetitive DNA sequences in the rat genome.

Entities: Gene Species

Mesh：

Substances：
DNA

Year: 1977 PMID： 270681 PMCID： PMC431946 DOI： 10.1073/pnas.74.10.4382

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

15 in total

5. An improved suppression subtractive hybridization technique to develop species-specific repetitive sequences from Erianthus arundinaceus (Saccharum complex).

Authors: Fan Yu; Yongji Huang; Ling Luo; Xueting Li; Jiayun Wu; Rukai Chen; Muqing Zhang; Zuhu Deng
Journal: BMC Plant Biol Date: 2018-11-06 Impact factor: 4.215

5 in total

Sequence relationship between long and short repetitive DNA of the rat: a preliminary report.

1. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.

2. Evolutionary divergence and length of repetitive sequences in sea urchin DNA.

3. Sequence organization of the human genome.

4. DNA sequence organization in the genomes of five marine invertebrates.

5. Exploration of long and short repetitive sequence relationships in the sea urchin genome.

6. A program for least squares analysis of reassociation and hybridization data.

Review 7. Organization, transcription, and regulation in the animal genome.

8. Arrangement and characterization of repetitive sequence elements in animal DNAs.

9. Characterization of macromolecules by constant velocity sedimentation.

10. The structure and evolution of ribosomal and 5S DNAs in Xenopus laevis and Xenopus mulleri.

1. On the complementarity of long repeated sequences in DNA to hnRNA.

Review 2. Sequence organization of animal nuclear DNA.

3. A family of long reiterated DNA sequences, one copy of which is next to the human beta globin gene.

4. Internal organization of long repetitive DNA sequences in sea urchin genomes.

5. An improved suppression subtractive hybridization technique to develop species-specific repetitive sequences from Erianthus arundinaceus (Saccharum complex).