Arash Azizi1, Zohreh Amirzadeh2, Maryam Rezai3, Shirin Lawaf4, Arash Rahimi5. 1. Oral Medicine department, Islamic Azad Universty-Dental Branch, Tehran, Iran. Electronic address: drarashazizi@yahoo.com. 2. Oral Medicine department, Islamic Azad Universty-Dental Branch, Tehran, Iran. Electronic address: Amirzadehzohreh@gmail.com. 3. Microbiology department, Islamic Azad University-Dental Branch, Tehran, Iran. Electronic address: maryamrezai_51@yahoo.com. 4. Prosthodontics department, Islamic Azad University-Dental Branch-Tehran-Iran. Electronic address: drshlawaf@yahoo.com. 5. Biophysics department, Islamic Azad University, Science and research Branch, Tehran, Iran. Electronic address: arash_dds@yahoo.com.
Abstract
BACKGROUND AND OBJECTIVES: Oral candidiasis (OC) is an opportunistic infection of the oral cavity most commonly caused by Candida albicans (C. albicans). Considering the drawbacks of standard treatments with antifungal agents, this study sought to assess the efficacy of photodynamic therapy (PDT) with methylene blue (MB) and indocyanine green (ICG) photosensitizers against C. albicans. MATERIALS AND METHODS: In this in-vitro, experimental study, 130 samples of C. albicans standard suspensions were subjected to various combinations of MB and ICG photosensitizers with and without laser irradiation with different exposure parameters, nystatin and chlorhexidine (CHX) in 13 groups of 10. Samples were cultured in microplates containing Sabouraud dextrose agar medium and colony forming units (CFUs) were counted after 24h of incubation at 37°C. Data were analyzed by SPSS version 19.0, one-way ANOVA and Tamhane's test. RESULTS: The maximum number of CFUs was seen in the control group (mean of 214,200CFUs with a log value of 5.32) while the minimum values were noted in the laser (808nm and 100Hz PRR) plus ICG (mean of 13,460CFUs and log value of 4.12) and nystatin (mean of 13,940CFUs and log value of 4.14) groups. CONCLUSION: Within the limitations of this in vitro study, the results revealed that laser application (808nm, 100Hz PRR) plus ICG caused a significant reduction in C. albicans CFUs.
BACKGROUND AND OBJECTIVES:Oral candidiasis (OC) is an opportunistic infection of the oral cavity most commonly caused by Candida albicans (C. albicans). Considering the drawbacks of standard treatments with antifungal agents, this study sought to assess the efficacy of photodynamic therapy (PDT) with methylene blue (MB) and indocyanine green (ICG) photosensitizers against C. albicans. MATERIALS AND METHODS: In this in-vitro, experimental study, 130 samples of C. albicans standard suspensions were subjected to various combinations of MB and ICG photosensitizers with and without laser irradiation with different exposure parameters, nystatin and chlorhexidine (CHX) in 13 groups of 10. Samples were cultured in microplates containing Sabouraud dextrose agar medium and colony forming units (CFUs) were counted after 24h of incubation at 37°C. Data were analyzed by SPSS version 19.0, one-way ANOVA and Tamhane's test. RESULTS: The maximum number of CFUs was seen in the control group (mean of 214,200CFUs with a log value of 5.32) while the minimum values were noted in the laser (808nm and 100Hz PRR) plus ICG (mean of 13,460CFUs and log value of 4.12) and nystatin (mean of 13,940CFUs and log value of 4.14) groups. CONCLUSION: Within the limitations of this in vitro study, the results revealed that laser application (808nm, 100Hz PRR) plus ICG caused a significant reduction in C. albicans CFUs.
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