Overexpression of a harpin-encoding gene popW from Ralstonia solanacearum primed antioxidant defenses with enhanced drought tolerance in tobacco plants.

KEY MESSAGE: The tobacco plants transformed with popW gene showed enhanced drought tolerance, and the mechanism was found with primed antioxidant defenses and reduced drought stress damages in the transgenic lines. Harpin proteins are elicitors produced by several gram-negative plant pathogenic bacteria, triggering multiple beneficial responses in plants, such as induction of defense response against diverse pathogens and insects, growth promotion, and drought tolerance. In this study, the harpin-encoding gene popW derived from Ralstonia solanacearum ZJ3721 was transferred to tobacco. We examined the tolerance of transgenic tobacco plants toward drought stress under greenhouse conditions and analyzed the molecular mechanisms underlying the enhanced drought tolerance. The results revealed that the transgenic lines primed antioxidant defenses and reduced drought stress damages. In addition, they displayed lower malondialdehyde and relative electrical conductivity, while higher relative water content and recovery intension than the tobacco plants transformed with empty vector pBI121 and the wild-type (WT) plants under drought stress. Furthermore, the transgenic lines displayed a significant increase in peroxidase, superoxide dismutase, catalase activities, and ascorbic acid content compared with control plants under drought stress, and these levels were up to 1.95, 1.68, 1.34, and 1.43 times higher than those of WT plants, respectively. Overexpression of popW in tobacco also significantly enhanced the relative transcript levels of oxidative stress-responsive genes NtAPX, NtCAT1, NtGST, and NtCu/Zn-SOD under drought stress. The relative transcript levels of these genes in the transgenic line PW12 were up to 1.94, 2.36, 5.24, and 3.62 times higher than those of WT plants, respectively. These results confirmed that the popW gene, which was transformed into tobacco primed antioxidant responses, increased tolerance to drought stress in tobacco plants.