Laura Iveth Miranda-Estrada1, María Ruíz-Rosas2, José Molina-López3, Isela Parra-Rojas1, Edgar González-Villalobos3, Natividad Castro-Alarcón4. 1. Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo de los Bravo, Guerrero, México. 2. Laboratorio Clínico, Clínica Hospital ISSSTE, Chilpancingo de los Bravo, Guerrero, México. 3. Laboratorio de Patogenicidad Bacteriana, Departamento de Salud Pública, Facultad de Medicina, UNAM, Ciudad de México, D.F., México; Hospital Infantil de México «Federico Gómez», Secretaría de Salud, Ciudad de México, D.F., México. 4. Laboratorio de Investigación en Microbiología, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo de los Bravo, Guerrero, México. Electronic address: natividadcastro24@gmail.com.
Abstract
INTRODUCTION: Escherichia coli is the major causative agent of urinary tract infections (UTI), and virulence factors are responsible for the severity of these emerging infections. The aim of this study was to evaluate the relationship between virulence determinants and antibiotic susceptibility with phylogenetic groups of E.coli isolates of UTI in two locations in Mexico. METHODS: An analysis was performed on 50 isolates of E.coli from the centre of the country and 57 from a town in the southwest. The isolates were characterized by phenotype (serotyping assays, in vitro adhesion, biofilm formation, production of haemolysin, and antibiotic susceptibility) and genotype (phylogenetic groups and virulence genes). RESULTS: In the centre of the country location the phylogenetic group B2 (60%) and F (12%) were significantly more prevalent and had a higher frequency of genes, fimH (96%), iutA (66%), sat (36%), compared to the southwest location, where the group A (35%) and B1 (21%) were significantly predominant and had fewer virulence genes. About one-fifth (21.5%) of all isolates belonged to the O25-ST131 group. Haemolysin and biofilm producing strains were significantly higher in the southwest location. Resistance to ampicillin (92.5%), tetracycline (76.6%), and trimethoprim/sulfamethoxazole (70.1%) were the most common in both groups. CONCLUSION: The phylogenetic group, virulence factors, and antibiotic susceptibility of the E.coli that causes UTI in the community, varies significantly among the Mexican populations studied. Phylogenetic groups A and B1 may be multidrug resistant and have the ability to produce UTI.
INTRODUCTION:Escherichia coli is the major causative agent of urinary tract infections (UTI), and virulence factors are responsible for the severity of these emerging infections. The aim of this study was to evaluate the relationship between virulence determinants and antibiotic susceptibility with phylogenetic groups of E.coli isolates of UTI in two locations in Mexico. METHODS: An analysis was performed on 50 isolates of E.coli from the centre of the country and 57 from a town in the southwest. The isolates were characterized by phenotype (serotyping assays, in vitro adhesion, biofilm formation, production of haemolysin, and antibiotic susceptibility) and genotype (phylogenetic groups and virulence genes). RESULTS: In the centre of the country location the phylogenetic group B2 (60%) and F (12%) were significantly more prevalent and had a higher frequency of genes, fimH (96%), iutA (66%), sat (36%), compared to the southwest location, where the group A (35%) and B1 (21%) were significantly predominant and had fewer virulence genes. About one-fifth (21.5%) of all isolates belonged to the O25-ST131 group. Haemolysin and biofilm producing strains were significantly higher in the southwest location. Resistance to ampicillin (92.5%), tetracycline (76.6%), and trimethoprim/sulfamethoxazole (70.1%) were the most common in both groups. CONCLUSION: The phylogenetic group, virulence factors, and antibiotic susceptibility of the E.coli that causes UTI in the community, varies significantly among the Mexican populations studied. Phylogenetic groups A and B1 may be multidrug resistant and have the ability to produce UTI.
Authors: Claudia A Freire; Ana Carolina M Santos; Antonio C Pignatari; Rosa M Silva; Waldir P Elias Journal: Braz J Microbiol Date: 2020-01-21 Impact factor: 2.476
Authors: Claudia A Freire; Rosa M Silva; Rita C Ruiz; Daniel C Pimenta; Jack A Bryant; Ian R Henderson; Angela S Barbosa; Waldir P Elias Journal: Front Immunol Date: 2022-02-17 Impact factor: 7.561
Authors: Manuel G Ballesteros-Monrreal; Margarita Mp Arenas-Hernández; Yessica Enciso-Martínez; Claudia F Martínez-de la Peña; Rosa Del C Rocha-Gracia; Patricia Lozano-Zaraín; Armando Navarro-Ocaña; Ygnacio Martínez-Laguna; Rafael de la Rosa-López Journal: Infect Drug Resist Date: 2020-01-30 Impact factor: 4.003
Authors: Gabriel Kambale Bunduki; Eva Heinz; Vincent Samuel Phiri; Patrick Noah; Nicholas Feasey; Janelisa Musaya Journal: BMC Infect Dis Date: 2021-08-04 Impact factor: 3.090