Charoenchai Puttipanyalears1,2, Nakarin Kitkumthorn3, Supranee Buranapraditkun4, Somboon Keelawat5, Apiwat Mutirangura2. 1. Inter-Department Program of BioMedical Sciences, Faculty of Graduate School, Chulalongkorn University, Bangkok, Thailand. 2. Center of Excellence in Molecular Genetics of Cancer & Human Diseases, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. 3. Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand. 4. Division of Allergy & Clinical Immunology, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. 5. Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Abstract
UNLABELLED: Changes in the methylation level of genes containing LINE-1 alter host gene regulation. AIM: This study demonstrates that paracrine signaling of breast cancer influences the epigenetic regulation of stromal cells. METHODS: We proved in vitro and in vivo breast cancer promoted LINE-1 methylation exists exclusively in female stromal cells. RESULTS: Genes containing LINE-1 of breast cancer stromal cells were upregulated. Furthermore, one of the genes, MUC-1, was demonstrated to have expression in plasma cells from the lymph nodes of patients with lymph node metastasis or micrometastasis. CONCLUSION: Breast cancer sends a paracrine signal to stroma cells causing LINE-1 epigenetic regulation. Moreover, the regulated genes in stroma cells are potential biomarkers for detecting breast cancer micrometastasis.
UNLABELLED: Changes in the methylation level of genes containing LINE-1 alter host gene regulation. AIM: This study demonstrates that paracrine signaling of breast cancer influences the epigenetic regulation of stromal cells. METHODS: We proved in vitro and in vivo breast cancer promoted LINE-1 methylation exists exclusively in female stromal cells. RESULTS: Genes containing LINE-1 of breast cancer stromal cells were upregulated. Furthermore, one of the genes, MUC-1, was demonstrated to have expression in plasma cells from the lymph nodes of patients with lymph node metastasis or micrometastasis. CONCLUSION:Breast cancer sends a paracrine signal to stroma cells causing LINE-1 epigenetic regulation. Moreover, the regulated genes in stroma cells are potential biomarkers for detecting breast cancer micrometastasis.
Entities:
Keywords:
LINE-1 hypermethylation; breast cancer; tumor supporting tissue
Authors: C Puttipanyalears; A Arayataweegool; K Chalertpet; P Rattanachayoto; P Mahattanasakul; N Tangjaturonsasme; V Kerekhanjanarong; A Mutirangura; N Kitkumthorn Journal: BMC Cancer Date: 2018-08-06 Impact factor: 4.430