| Literature DB >> 2703257 |
M Steffen1, M Abboud, G K Potter, Y P Yung, M A Moore.
Abstract
The observation that mast cell products and cachectin/tumour necrosis factor (TNF) mediate similar responses suggested an investigation of cultured human basophil/mast cells for production of TNF. Using in situ hybridization and the avidin biotin-complex (ABC) immunoperoxidase method, we have demonstrated the presence of TNF mRNA in the cytoplasm and TNF protein in the granules of individual human basophil/mast cells. The production of TNF by these cells could explain many of their reported functions.Entities:
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Year: 1989 PMID: 2703257 PMCID: PMC1385235
Source DB: PubMed Journal: Immunology ISSN: 0019-2805 Impact factor: 7.397