Literature DB >> 2703238

Detection of single-copy genes by nonisotopic in situ hybridization on human chromosomes.

D Cherif1, O Bernard, R Berger.   

Abstract

A technique of in situ hybridization on metaphase chromosomes with biotinylated DNA probes is described. This technique was used to localize unique DNA sequences on chromosomes and allowed a localization of two probes 1.8 and 1.3 kb long. The hybridization signal appears like two, twin, spots on the two sister chromatids, allowing a clear distinction from the background. Moreover a chromosomal localization is possible by counting a relatively small number of mitoses compared with the technique using 3H-labeled DNA probes.

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Year:  1989        PMID： 2703238     DOI： 10.1007/BF00283691

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  20 in total

1.  "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1984-02       Impact factor: 3.365

2.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

3.  A simple method of reducing the fading of immunofluorescence during microscopy.

Authors:  G D Johnson; G M Nogueira Araujo
Journal:  J Immunol Methods       Date:  1981       Impact factor: 2.303

4.  Localization of a unique gene by direct hybridization in situ.

Authors:  D S Gerhard; E S Kawasaki; F C Bancroft; P Szabo
Journal:  Proc Natl Acad Sci U S A       Date:  1981-06       Impact factor: 11.205

5.  Selection of cells with different chromosomal localizations of the amplified c-myc gene during in vivo and in vitro growth of the breast carcinoma cell line SW 613-S.

Authors:  D Cherif; C Lavialle; N Modjtahedi; M Le Coniat; R Berger; O Brison
Journal:  Chromosoma       Date:  1989-01       Impact factor: 4.316

6.  Chromosomal localization of the human proto-oncogene c-ets.

Authors:  C de Taisne; A Gegonne; D Stehelin; A Bernheim; R Berger
Journal:  Nature       Date:  1984 Aug 16-22       Impact factor: 49.962

7.  Chromosomal localization of a unique gene by non-autoradiographic in situ hybridization.

Authors:  J E Landegent; N Jansen in de Wal; G J van Ommen; F Baas; J J de Vijlder; P van Duijn; M Van der Ploeg
Journal:  Nature       Date:  1985 Sep 12-18       Impact factor: 49.962

8.  Conserved chromosomal positions of dual domains of the ets protooncogene in cats, mice, and humans.

Authors:  D K Watson; M J McWilliams-Smith; C Kozak; R Reeves; J Gearhart; M F Nunn; W Nash; J R Fowle; P Duesberg; T S Papas
Journal:  Proc Natl Acad Sci U S A       Date:  1986-03       Impact factor: 11.205

9.  Use of whole cosmid cloned genomic sequences for chromosomal localization by non-radioactive in situ hybridization.

Authors:  J E Landegent; N Jansen in de Wal; R W Dirks; F Baao; M van der Ploeg
Journal:  Hum Genet       Date:  1987-12       Impact factor: 4.132

10.  Mapping muscle protein genes by in situ hybridization using biotin-labeled probes.

Authors:  D G Albertson
Journal:  EMBO J       Date:  1985-10       Impact factor: 11.598
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  14 in total

Review 1.  Interphase cytogenetics.

Authors:  C S Herrington; J O McGee
Journal:  Neurochem Res       Date:  1990-04       Impact factor: 3.996

2.  Mapping small DNA sequences by fluorescence in situ hybridization directly on banded metaphase chromosomes.

Authors:  Y S Fan; L M Davis; T B Shows
Journal:  Proc Natl Acad Sci U S A       Date:  1990-08       Impact factor: 11.205

3.  Simultaneous localization of cosmids and chromosome R-banding by fluorescence microscopy: application to regional mapping of human chromosome 11.

Authors:  D Cherif; C Julier; O Delattre; J Derré; G M Lathrop; R Berger
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

4.  Simultaneous visualization of chromosome bands and hybridization signal using colloidal-gold labeling in electron microscopy.

Authors:  R Fetni; R Drouin; N Lemieux; P E Messier; C L Richer
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-01       Impact factor: 11.205

5.  Three-dimensional organization of the ribosomal genes and Ag-NOR proteins during interphase and mitosis in PtK1 cells studied by confocal microscopy.

Authors:  I Robert-Fortel; H R Junéra; G Géraud; D Hernandez-Verdun
Journal:  Chromosoma       Date:  1993-02       Impact factor: 4.316

6.  Interphase cytogenetics in paraffin sections of lung tumors by non-isotopic in situ hybridization. Mapping genotype/phenotype heterogeneity.

Authors:  S Y Kim; J S Lee; J Y Ro; M L Gay; W K Hong; W N Hittelman
Journal:  Am J Pathol       Date:  1993-01       Impact factor: 4.307

Review 7.  Enzymatic labeling of nucleic acids.

Authors:  J Temsamani; S Agrawal
Journal:  Mol Biotechnol       Date:  1996-06       Impact factor: 2.695

8.  Differential distribution of long and short interspersed element sequences in the mouse genome: chromosome karyotyping by fluorescence in situ hybridization.

Authors:  A L Boyle; S G Ballard; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

9.  Direct carrier detection by in situ suppression hybridization with cosmid clones of the Duchenne/Becker muscular dystrophy locus.

Authors:  T Ried; V Mahler; P Vogt; L Blonden; G J van Ommen; T Cremer; M Cremer
Journal:  Hum Genet       Date:  1990-10       Impact factor: 4.132

10.  Chromosomal locations of four minor rDNA loci and a marker microsatellite sequence in barley.

Authors:  C Pedersen; I Linde-Laursen
Journal:  Chromosome Res       Date:  1994-01       Impact factor: 5.239
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