| Literature DB >> 27028517 |
Qifeng Zhou1, Jing Zheng1, Zhihe Qing2, Mengjie Zheng1, Jinfeng Yang1, Sheng Yang2, Le Ying1, Ronghua Yang2.
Abstract
The levels of circulating tumor DNA (ctDNA) in the peripheral blood have been associated with tumor burden and malignant progression. However, ultrasensitive detection of ctDNA in blood remains to be explored. Herein, we have developed a new approach, employing DNA-mediated surface-enhanced Raman scattering (SERS) of single-walled carbon nanotubes (SWNTs), that allows ultrasensitive detection of a broad range of ctDNAs in human blood. Combined with the efficient ctDNA recognition capacity of our designed triple-helix molecular switch and RNase HII enzyme-assisted amplification, the T-rich DNA-mediated SERS enhancement of SWNTs could read out a content of KRAS G12DM as low as 0.3 fM, with a detection of 5.0 μL of sample volume, which has potential for point-of-care testing in clinical analysis.Entities:
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Year: 2016 PMID： 27028517 DOI： 10.1021/acs.analchem.6b00108
Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986