| Literature DB >> 27008570 |
Christopher R Drake1, Natalia Sevillano2, Charles Truillet1, Charles S Craik2, Henry F VanBrocklin1, Michael J Evans1.
Abstract
New methodologies for site-specifically radiolabeling proteins with (18)F are required to generate high quality radiotracers for preclinical and clinical applications with positron emission tomography. Herein, we report an approach by which we use lipoic acid ligase (LplA) to conjugate [(18)F]-fluorooctanoic acid to an antibody fragment bearing the peptide substrate of LplA. The mild conditions of the reaction preserve antibody immunoreactivity, and the efficiency of LplA allows for >90% yield even with very small amounts of peptidic precursor (1-10 nmol). These features are advantageous compared to the current gold standard in the field. Moreover, the methodology introduces a new application for an important tool in chemical biology.Entities:
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Year: 2016 PMID： 27008570 PMCID： PMC5712215 DOI： 10.1021/acschembio.6b00172
Source DB: PubMed Journal: ACS Chem Biol ISSN： 1554-8929 Impact factor: 5.100