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Literature DB >> 26998273

4-Nitroquinoline-1-oxide effects human lung adenocarcinoma A549 cells by regulating the expression of POLD4.

Qin-Miao Huang¹, Yi-Ming Zeng¹, Hua-Ping Zhang¹, Liang-Chao Lv¹, Dong-Yong Yang¹, Hui-Huang Lin¹.

Abstract

The aim of the present study was to explore the expression of POLD4 in human lung adenocarcinoma A549 cells under 4-nitroquinoline-1-oxide (4NQO) stimulation to investigate the role of POLD4 in smoking-induced lung cancer. The lung cancer A549 cell line was treated with 4NQO, with or without MG132 (an inhibitor of proteasome activity), and subsequently the POLD4 level was determined by western blot analysis. Secondly, the cell sensitivity to 4NQO and Taxol was determined when the POLD4 expression level was downregulated by siRNA. The POLD4 protein levels in the A549 cells decreased following treatment with 4NQO; however, MG132 could reverse this phenotype. Downregulation of the POLD4 expression by siRNA enhanced A549 cell sensitivity to 4NQO, but not to Taxol. In conclusion, 4NQO affects human lung adenocarcinoma A549 cells by regulating the expression of POLD4.

Entities: Chemical Disease Gene Species

Keywords: 4-nitroquinoline-1-oxide; POLD4; Taxol; lung cancer; role; smoking

Year: 2016 PMID： 26998273 PMCID： PMC4774375 DOI： 10.3892/br.2016.583

Source DB: PubMed Journal: Biomed Rep ISSN： 2049-9434

17 in total

1. Identification of RNF8 as a ubiquitin ligase involved in targeting the p12 subunit of DNA polymerase δ for degradation in response to DNA damage.

Authors: Sufang Zhang; Yajing Zhou; Ali Sarkeshik; John R Yates; Timothy M Thomson; Zhongtao Zhang; Ernest Y C Lee; Marietta Y W T Lee
Journal: J Biol Chem Date: 2012-12-11 Impact factor: 5.157

4-Nitroquinoline-1-oxide effects human lung adenocarcinoma A549 cells by regulating the expression of POLD4.

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Review 7. Tobacco carcinogens, their biomarkers and tobacco-induced cancer.

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