OBJECTIVES: This study aimed to investigate the influence of hypoxia on angiogenesis in a 3D gel, with co-culturing adipose-derived stromal cells (ASCs) and endothelial cells (ECs). MATERIALS AND METHODS: ASCs from green fluorescent protein-labeled mice and ECs from red fluorescent protein-labeled mice were co-cultured in 3D collagen gels at 1:1 ratio, in normal and hypoxic oxygen conditions, and morphology of angiogenesis was observed using confocal laser scanning microscopy. To discover changes in growth factors between monoculture ASCs and ECs, transwell co-cultures of ASCs and ECs were applied. Semi-quantitative PCR was performed to explore mRNA expression of growth factors. RESULTS: Enhanced angiogenesis was observed in 3D gels implanted with 1:1 mixture of ASCs and ECs after 7 days hypoxia. Genes including VEGFA/B, EGF-1, HIF-1a, IGF-1, PDGF, TGF-β1 and BMP-2/4 in ECs, both monoculture and co-culture, were significantly enhanced after being cultured under hypoxia. In comparison, genes VEGFA/B, EGF-1, HIF-1a, TGF-β1 and BMP-2 in ASCs increased. In all, factors VEGFA/B, EGF-1, HIF-1a, TGF-β1 and BMP-2 increased in both ASCs and ECs after being cultured in hypoxia no matter whether as monoculture or co-culture. CONCLUSIONS: Co-culture of ASCs and ECs at 1:1 ratio in a 3D gel under hypoxia promoted angiogenesis. Those growth factors which were increased in both ASCs and ECs, indicate that VEGFA/B, EGF-1, HIF-1a, TGF-β1 and BMP-2 might be responsible for enhancement in angiogenesis triggered by hypoxia.
OBJECTIVES: This study aimed to investigate the influence of hypoxia on angiogenesis in a 3D gel, with co-culturing adipose-derived stromal cells (ASCs) and endothelial cells (ECs). MATERIALS AND METHODS: ASCs from green fluorescent protein-labeled mice and ECs from red fluorescent protein-labeled mice were co-cultured in 3D collagen gels at 1:1 ratio, in normal and hypoxic oxygen conditions, and morphology of angiogenesis was observed using confocal laser scanning microscopy. To discover changes in growth factors between monoculture ASCs and ECs, transwell co-cultures of ASCs and ECs were applied. Semi-quantitative PCR was performed to explore mRNA expression of growth factors. RESULTS: Enhanced angiogenesis was observed in 3D gels implanted with 1:1 mixture of ASCs and ECs after 7 days hypoxia. Genes including VEGFA/B, EGF-1, HIF-1a, IGF-1, PDGF, TGF-β1 and BMP-2/4 in ECs, both monoculture and co-culture, were significantly enhanced after being cultured under hypoxia. In comparison, genes VEGFA/B, EGF-1, HIF-1a, TGF-β1 and BMP-2 in ASCs increased. In all, factors VEGFA/B, EGF-1, HIF-1a, TGF-β1 and BMP-2 increased in both ASCs and ECs after being cultured in hypoxia no matter whether as monoculture or co-culture. CONCLUSIONS: Co-culture of ASCs and ECs at 1:1 ratio in a 3D gel under hypoxia promoted angiogenesis. Those growth factors which were increased in both ASCs and ECs, indicate that VEGFA/B, EGF-1, HIF-1a, TGF-β1 and BMP-2 might be responsible for enhancement in angiogenesis triggered by hypoxia.
Authors: Kimio Takeuchi; Ryoji Yanai; Fumiaki Kumase; Yuki Morizane; Jun Suzuki; Maki Kayama; Katarzyna Brodowska; Mitsuru Nakazawa; Joan W Miller; Kip M Connor; Demetrios G Vavvas Journal: PLoS One Date: 2014-03-19 Impact factor: 3.240
Authors: Javier Fernández-Torres; Gabriela Angélica Martínez-Nava; Yessica Zamudio-Cuevas; Karina Martínez-Flores; María Concepción Gutiérrez-Ruíz; Luis Enrique Gómez-Quiroz; Daniela Garrido-Rodríguez; José Francisco Muñoz-Valle; Edith Oregón-Romero; Carlos Lozada; Denise Clavijo Cornejo; Carlos Pineda; Alberto López-Reyes Journal: Clin Rheumatol Date: 2019-06-25 Impact factor: 2.980
Authors: William J Wulftange; Michelle A Rose; Marcial Garmendia-Cedillos; Davi da Silva; Joanna E Poprawski; Dhruv Srinivasachar; Taylor Sullivan; Langston Lim; Valery V Bliskovsky; Matthew D Hall; Thomas J Pohida; Robert W Robey; Nicole Y Morgan; Michael M Gottesman Journal: J Cell Physiol Date: 2019-04-22 Impact factor: 6.384
Authors: Arno Amann; Marit Zwierzina; Stefan Koeck; Gabriele Gamerith; Elisabeth Pechriggl; Julia M Huber; Edith Lorenz; Jens M Kelm; Wolfgang Hilbe; Heinz Zwierzina; Johann Kern Journal: Sci Rep Date: 2017-06-07 Impact factor: 4.379