Fatemeh Vahedi1, Elnaz Ghorbani2, Tahereh Falsafi2. 1. Razi Vaccine and Serum Research Institute, Mashhad, Iran. 2. Microbiology Department, Al-Zahra University, Tehran, Iran.
Abstract
BACKGROUND: DNA vaccination with plasmid encoding bacterial, viral, and parasitic immunogens has been shown to be an attractive method to induce efficient immune responses. Bacteria of the genus Brucella are facultative intracellular pathogens for which new and efficient vaccines are needed. METHODS: To evaluate the use of a DNA immunization strategy for protection against brucellosis, a plasmid containing the DNA encoding the Brucella melitensis (B. melitensis) 31 kDa outer membrane protein, as a potent immunogenic target, was constructed. RESULTS: The constructed plasmid, pcDNA3.1+omp31, was injected intramuscularly into mice and the expression of omp31 RNA was assessed by RT-PCR. The integrity of the pcDNA3.1+omp31 construct was confirmed with restriction analysis and sequencing. Omp31 mRNA expression was verified by RT-PCR. CONCLUSION: Our results indicate that the pcDNA3.1+omp31 eukaryotic expression vector expresses omp31 mRNA and could be useful as a vaccine candidate.
BACKGROUND: DNA vaccination with plasmid encoding bacterial, viral, and parasitic immunogens has been shown to be an attractive method to induce efficient immune responses. Bacteria of the genus Brucella are facultative intracellular pathogens for which new and efficient vaccines are needed. METHODS: To evaluate the use of a DNA immunization strategy for protection against brucellosis, a plasmid containing the DNA encoding the Brucella melitensis (B. melitensis) 31 kDa outer membrane protein, as a potent immunogenic target, was constructed. RESULTS: The constructed plasmid, pcDNA3.1+omp31, was injected intramuscularly into mice and the expression of omp31 RNA was assessed by RT-PCR. The integrity of the pcDNA3.1+omp31 construct was confirmed with restriction analysis and sequencing. Omp31 mRNA expression was verified by RT-PCR. CONCLUSION: Our results indicate that the pcDNA3.1+omp31 eukaryotic expression vector expresses omp31 mRNA and could be useful as a vaccine candidate.
Entities:
Keywords:
Brucella melitensis; DNA Vaccine; omp31; pcDNA3.1
Authors: Sophie Leclerq; Jerome S Harms; Gracia M S Rosinha; Vasco Azevedo; Sergio C Oliveira Journal: J Med Microbiol Date: 2002-01 Impact factor: 2.472
Authors: A Al-Mariri; A Tibor; P Mertens; X De Bolle; P Michel; J Godfroid; K Walravens; J J Letesson Journal: Infect Immun Date: 2001-10 Impact factor: 3.441
Authors: R Adone; F Ciuchini; C Marianelli; M Tarantino; C Pistoia; G Marcon; P Petrucci; M Francia; G Riccardi; P Pasquali Journal: Infect Immun Date: 2005-07 Impact factor: 3.441
Authors: Carlos A Velikovsky; Juliana Cassataro; Guillermo H Giambartolomei; Fernando A Goldbaum; Silvia Estein; Raul A Bowden; Laura Bruno; Carlos A Fossati; Moisés Spitz Journal: Infect Immun Date: 2002-05 Impact factor: 3.441
Authors: P Lestrate; R M Delrue; I Danese; C Didembourg; B Taminiau; P Mertens; X De Bolle; A Tibor; C M Tang; J J Letesson Journal: Mol Microbiol Date: 2000-11 Impact factor: 3.501
Authors: Mireille M Kattar; Rola F Jaafar; George F Araj; Philippe Le Flèche; Ghassan M Matar; Roland Abi Rached; Simon Khalife; Gilles Vergnaud Journal: J Clin Microbiol Date: 2008-10-15 Impact factor: 5.948
Authors: Silvia M Estein; María A Fiorentino; Fernando A Paolicchi; María Clausse; Jorge Manazza; Juliana Cassataro; Guillermo H Giambartolomei; Lorena M Coria; Vanesa Zylberman; Carlos A Fossati; Rune Kjeken; Fernando A Goldbaum Journal: Vaccine Date: 2009-09-11 Impact factor: 3.641