Tricia Murielly Pereira Andrade de Souza1, Ricardo Dias de Castro2, Laís César de Vasconcelos1, Andréa Dos Anjos Pontual3, Flávia Maria de Moraes Ramos Perez3, Maria Luiza Dos Anjos Pontual4. 1. Federal University of Pernambuco (UFPE), Av. Professor Morais Rego, 1235, Cidade Universitária, Recife, Recife, Pernambuco, 50670-901, Brazil. 2. Phonoaudiology Department, Federal University of Paraíba, Cidade Universitária, - Castelo Branco, João Pessoa, Paraiba, João Pessoa, 58051-900, Brazil. 3. Department of Clinical and Preventive Dentistry, Federal University of Pernambuco, Av. Professor Morais Rego, 1235 - Cidade Universitária, Recife, Recife, Pernambuco, 50670-901, Brazil. 4. Department of Clinical and Preventive Dentistry, Federal University of Pernambuco, Av. Professor Morais Rego, 1235 - Cidade Universitária, Recife, Recife, Pernambuco, 50670-901, Brazil. mlpontual@gmail.com.
Abstract
OBJECTIVES: The aims of this study were to evaluate microbial contamination in phosphor storage plates in dental radiology services and discuss the possible origin of this contamination. MATERIALS AND METHODS: The sample comprised 50 phosphor plates: 14 plates from service A, 30 from service B, and 6 in the control group, consisting of plates never used. Damp sterile swabs were rubbed on the phosphor plates, and then transferred to tests tubes containing sterile saline solution. Serial dilutions were made, and then inoculated in triplicate on Mueller Hinton agar plates and incubated at 37 °C/48 h, before counting the colony-forming units (CFU). The samples were also seeded in brain-heart infusion medium to confirm contamination by turbidity of the culture medium. All solutions, turbid and clean, were seeded in selective and non-selective media. RESULTS: At service A and B, 50 and 73.3 % of the phosphor plates were contaminated, respectively. This contamination was mainly due to bacteria of the genus Staphylococcus. CFU counts ranged from 26.4 to 80.0 CFU/plate. CONCLUSIONS: Most of the phosphor plates evaluated shown to be contaminated, mainly by Staphylococcus ssp. Quantitatively, this contamination occurred at low levels, possibly arising from handling of the plates. The use of a second plastic barrier may have diminished contamination by microorganisms from the oral cavity. CLINICAL RELEVANCE: There is a risk of cross-contamination by phosphor storage plates used in dental radiology services.
OBJECTIVES: The aims of this study were to evaluate microbial contamination in phosphor storage plates in dental radiology services and discuss the possible origin of this contamination. MATERIALS AND METHODS: The sample comprised 50 phosphor plates: 14 plates from service A, 30 from service B, and 6 in the control group, consisting of plates never used. Damp sterile swabs were rubbed on the phosphor plates, and then transferred to tests tubes containing sterile saline solution. Serial dilutions were made, and then inoculated in triplicate on Mueller Hinton agar plates and incubated at 37 °C/48 h, before counting the colony-forming units (CFU). The samples were also seeded in brain-heart infusion medium to confirm contamination by turbidity of the culture medium. All solutions, turbid and clean, were seeded in selective and non-selective media. RESULTS: At service A and B, 50 and 73.3 % of the phosphor plates were contaminated, respectively. This contamination was mainly due to bacteria of the genus Staphylococcus. CFU counts ranged from 26.4 to 80.0 CFU/plate. CONCLUSIONS: Most of the phosphor plates evaluated shown to be contaminated, mainly by Staphylococcus ssp. Quantitatively, this contamination occurred at low levels, possibly arising from handling of the plates. The use of a second plastic barrier may have diminished contamination by microorganisms from the oral cavity. CLINICAL RELEVANCE: There is a risk of cross-contamination by phosphor storage plates used in dental radiology services.
Entities:
Keywords:
Dental digital radiography; Dental radiography; Equipment contamination; Infection control
Authors: Sajitha Kalathingal; Alison Youngpeter; Jason Minton; Michael Shrout; Douglas Dickinson; Kevin Plummer; Stephen Looney Journal: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Date: 2010-01-08
Authors: Marco Antônio Lavorato de Almeida; André Ulisses Dantas Batista; Maria Rejane Cruz de Araújo; Vanessa Fabiana Dei Santi de Almeida; Paulo Rogério Ferreti Bonan; Danielle Nóbrega Alves; Tereza Karla Vieira Lopes da Costa; Diego Figueiredo Nóbrega; Ricardo Dias de Castro Journal: BMC Oral Health Date: 2020-08-17 Impact factor: 2.757