Literature DB >> 2697743

Polymerase chain reaction for the detection of Mycobacterium leprae.

R A Hartskeerl1, M Y de Wit, P R Klatser.   

Abstract

A polymerase chain reaction (PCR) using heat-stable Taq polymerase is described for the specific detection of Mycobacterium leprae, the causative agent of leprosy. A set of primers was selected on the basis of the nucleotide sequence of a gene encoding the 36 kDa antigen of M. leprae. With this set of primers in the PCR, M. leprae could be detected specifically with a detection limit approximating one bacterium. This PCR appears to meet the criteria of specificity and sensitivity required for a useful tool in epidemiology and eventually for the control of leprosy.

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Year:  1989        PMID： 2697743     DOI： 10.1099/00221287-135-9-2357

Source DB:  PubMed          Journal:  J Gen Microbiol        ISSN： 0022-1287


  41 in total

1.  Characterization of natural populations of Nitrobacter spp. using PCR/RFLP analysis of the ribosomal intergenic spacer.

Authors:  E Navarro; P Simonet; P Normand; R Bardin
Journal:  Arch Microbiol       Date:  1992       Impact factor: 2.552

2.  Purification of sputum samples through sucrose improves detection of Mycobacterium tuberculosis by polymerase chain reaction.

Authors:  T Victor; R du Toit; P D van Helden
Journal:  J Clin Microbiol       Date:  1992-06       Impact factor: 5.948

3.  Diagnostic value of in situ Polymerase Chain Reaction in leprosy.

Authors:  R Dayal; S P Singh; P P Mathur; V M Katoch; K Katoch; M Natrajan
Journal:  Indian J Pediatr       Date:  2005-12       Impact factor: 1.967

4.  PCR and in-situ hybridization for diagnosis of leprosy.

Authors:  R Dayal; M Agarwal; M Natrajan; V M Katoch; K Katoch; Kalpna Singh; D S Chauhan
Journal:  Indian J Pediatr       Date:  2007-07       Impact factor: 1.967

5.  Detection of untreated mycobacteria by using polymerase chain reaction and specific DNA probes.

Authors:  J W Fries; R J Patel; W F Piessens; D F Wirth
Journal:  J Clin Microbiol       Date:  1991-08       Impact factor: 5.948

6.  Detection of enterotoxigenic Staphylococcus aureus in dried skimmed milk: use of the polymerase chain reaction for amplification and detection of staphylococcal enterotoxin genes entB and entC1 and the thermonuclease gene nuc.

Authors:  I G Wilson; J E Cooper; A Gilmour
Journal:  Appl Environ Microbiol       Date:  1991-06       Impact factor: 4.792

7.  Detection of Mycobacterium leprae infection by PCR.

Authors:  J Wichitwechkarn; S Karnjan; S Shuntawuttisettee; C Sornprasit; K Kampirapap; S Peerapakorn
Journal:  J Clin Microbiol       Date:  1995-01       Impact factor: 5.948

8.  Discrimination of Mycobacterium leprae and Mycobacterium haemophilum in Clinical Isolates and Specimens by Multiplex PCR Assay and Prediction of Drug Susceptibility.

Authors:  Naoya Kitaoka; Hanako Fukano; Mitsunori Yoshida; Yuji Miyamoto; Shuichi Mori; Norihisa Ishii; Manabu Ato; Naoya Ohara; Yoshihiko Hoshino
Journal:  J Clin Microbiol       Date:  2019-01-30       Impact factor: 5.948

9.  Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease.

Authors:  P H Vary; P R Andersen; E Green; J Hermon-Taylor; J J McFadden
Journal:  J Clin Microbiol       Date:  1990-05       Impact factor: 5.948

10.  Amplification of a species-specific DNA fragment of Mycobacterium tuberculosis and its possible use in diagnosis.

Authors:  P Del Portillo; L A Murillo; M E Patarroyo
Journal:  J Clin Microbiol       Date:  1991-10       Impact factor: 5.948
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