| Literature DB >> 26976882 |
Elisa H P Andrade1, Leandra B Figueiredo1, Ana P P Vilela1, Júlio C C Rosa1, Jaquelline G Oliveira1, Hassan M Zibaoui1, Valdelaine E M Araújo1, Daniela P J Miranda1, Paulo C P Ferreira1, Jonatas S Abrahão1, Erna G Kroon2.
Abstract
Dengue is currently regarded as a major public health problem worldwide. In a hyperendemic region during an outbreak, we detected the co-circulation of all Dengue virus (DENV) serotypes including two different genotypes of DENV-3 and DENV-4, and concurrent infections with up to three serotypes were identified in symptomatic patients. A total of 49 acute phase plasma samples from patients clinically suspected of dengue were collected during the 4 weeks of May 2013. DENV-1-4 was detected by reverse transcriptase semi-nested polymerase chain reaction in 33 samples (67.3%), of which 26 DNA fragments were sequenced. Twenty samples (76.9%) were identified with a single DENV serotype and six (23.1%) with more than one serotype. DENV-3 was the predominant serotype of the outbreak. On the basis of phylogenetic analyses, DENV-1 isolates belong to genotype V, DENV-2 to American-Asian genotype, DENV-3 to genotypes I and III, and DENV-4 to genotypes I and II. © The American Society of Tropical Medicine and Hygiene.Entities:
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Year: 2016 PMID： 26976882 PMCID： PMC4856606 DOI： 10.4269/ajtmh.15-0892
Source DB: PubMed Journal: Am J Trop Med Hyg ISSN： 0002-9637 Impact factor: 2.345