Comparison of Anthropometric Data Between Asian and Caucasian Patients With Obstructive Sleep Apnea: A Meta-Analysis.

OBJECTIVES: Obesity is considered to be one of the most important risk factors for obstructive sleep apnea (OSA) but less is known about the role of ethnicity in OSA. The purpose of this study was to investigate the interethnic difference of obesity-related phenotypes in OSA and to reveal the role of ethnicity in OSA.
METHODS: We searched MEDLINE, LILACS, Scopus, and the Cochrane Library using the key words "sleep apnea," "body mass index," "neck circumference," "waist circumference," "waist to hip ratio," etc. Inclusion criteria were adults over 18 years of age, and studies that included polysomnography, obesity-related parameters, and a clear demarcation of ethnicity in the patient population. Included studies were reviewed by 2 independent reviewers. The following information was collected for controls and OSA: number, age, gender, country, ethnicity (Asian or Caucasian), study design, apnea-hypopnea index/respiratory disturbance index, body mass index (BMI), neck circumference (NC), waist circumference (WC), and/or waist to hip ratio (WHR).
RESULTS: A total of 8,312 publications were retrieved with a subsequent 19 manuscripts that met the selection criteria. A total of 2,966 patients were included for analysis. The main findings were as follows: There was no difference in BMI, WC, and WHR between patients with OSA and controls after accounting for publication bias; Patients with OSA have greater NC than controls (standard mean difference, 0.89; 95% confidence interval, 0.63 to 1.14); and There was no difference in NC between Asian and Caucasians patients (P=0.178).
CONCLUSION: OSA might not be related with BMI, WC, and WHR. Only NC demonstrated a strong association with OSA, and this finding was not different between Asians and Caucasians.

INTRODUCTION

Obstructive sleep apnea (OSA) is a common disease worldwide, affecting over 4% of men and 2% of women [1]. It is characterized by repetitive upper airway collapse during sleep, which induces frequent arousals and oxygen desaturation. OSA is associated with a variety of symptoms and conditions such as fatigue, daytime somnolence, headache, myocardial infarction, arrhythmias, stroke, and an increased incidence of motor vehicle accidents. Therefore, accurate diagnosis and treatment of OSA is recommended to improve quality of life and to reduce the risk of associated morbidity and mortality.

Numerous studies report that OSA is closely related with obesity, which is thought to reduce the diameter of upper airway and increase its collapsibility [2,3]. Several anthropometric measurements like body mass index (BMI), neck circumference (NC), waist circumference (WC), and waist-hip ratio (WHR) have been frequently used to investigate the association between obesity and OSA [4-22]. However, the results have varied based on age, gender, and ethnicity.

Asian patients with OSA are suspected to be affected to a greater degree by obesity than Caucasians [23,24]. However, this finding is not conclusive because large-scale comparisons between different ethnicities are difficult to perform at a single research center. The goal of this study was to provide a systematic and critical appraisal of the available evidence on the anthropometric differences between Asian and Caucasian patients with OSA. Understanding the different obesity phenotypes between Asian and Caucasian may help to elucidate the underlying mechanisms of OSA, provide important clinical tools for screening of OSA, and have an effect on selecting treatment modalities.

MATERIALS AND METHODS

Literature sources and study identification

This study was designed to compare the obesity-related parameters including BMI, NC, WC, and WHR between Asian and Caucasian patients with and without OSA. We searched electronic databases including MEDLINE, LILACS, Scopus, and the Cochrane Library to identify relevant articles published in English from 1970 to October 2013. The following key words were used: sleep apnea AND body mass index; sleep apnea AND neck circumference; sleep apnea AND waist circumference; sleep apnea AND waist to hip ratio; sleep apnea AND central obesity; sleep apnea AND anthropometry. Only manuscripts published in English were eligible. Reviews, guidelines, letters, case reports, editorials, in vitro and animal studies were excluded. Data was extracted from the final group of included studies using a standardized datasheet. This was performed independently in duplicate (by authors JHC and SHC) and all authors reconciled the results.

Eligibility criteria

All case-control, cross-sectional, and cohort studies were included if the study population was limited to adults older than 18 years, a laboratory polysomnography or home sleep test was performed, obesity-related parameters (BMI, NC, WC, and/or WHR) were measured, and there was a clear demarcation of ethnicity, especially in countries with mixed ethnic populations such as in United States. We excluded studies which did not provide raw data on apnea-hypopnea index (AHI)/respiratory disturbance index (RDI) and obesity-related parameters (BMI, NC, WC, and/or WHR) with mean and standard deviations. We also excluded studies which included cases of pregnancy, craniofacial deformity, and bariatric surgery.

Data collection

For each study, the following information was collected: number of patients and controls, age, gender, country, ethnicity (Asian or Caucasian), study design, AHI/RDI, BMI, NC, WC, and/or WHR. According to the map of ethnic distribution based on genetic similarity, we classified materials into 2 ethnic groups; 9 Asians (Korea, China, Japan, and Thailand) and 10 Caucasians (United States, Italy, Spain, Turkey, and India) [25]. If two or more studies presented the same data from a single patient population, we included the data in our analysis only once.

Statistical analyses

BMI, NC, WC, and WHR were statistically analyzed totally and separately based on ethnicity, specifically between Asian and Caucasian groups. We used the random-effects model according to Dersimonian-Laird and the fixed effects model according to the inverse variance approach simultaneously irrespective of heterogeneity, and provided both results. Heterogeneity was calculated with the Cochran’s Q statistic test and the I2 test. We considered an I2 value greater than 50% and P<0.05 as indicative of heterogeneity. Subgroup data was compared to explore the differences between Asian and Caucasian. If the heterogeneity existed between groups, we considered that there was difference between the two. Publication bias was examined by visual inspection of a funnel plot and Egger’s test for bias [26]. Publication bias is suspected when the funnel plot is asymmetrical. We also performed the Duval and Tweedie “trim-and-fill” procedure to further assess the possible effect of publication bias in our meta-analysis [27]. Analysis was performed using STATA ver. 13 (StataCorp LP., College Station, TX, USA). All results are reported with standard mean difference (SMD) and 95% confidence intervals (CIs). All P-values were two-tailed.

Search results and characteristics

A total of 8,312 manuscripts were retrieved. A systematic review of titles, abstracts, and full text publications was carried out. 8,260 studies were discarded and 52 studies were selected for the further review. Thirty-three studies were abandoned due to lack of raw data or discordance of inclusion criteria. Finally, 19 studies remained for data extraction. The search results are summarized in Fig. 1.

Fig. 1.

Search results.

RESULTS

This meta-analysis included 19 studies consisting of data from a total of 2,966 patients (803 controls and 2,163 with OSA, Table 1). Males represented 57.9% of controls (range, 8.3% to 90%), and 76.5% of patients with OSA (range, 40.4% to 90%). For controls and OSA group, there was no statistical difference in sex and age between Asians and Caucasians. Sixteen studies (84.2%) defined the presence of OSA with the criteria of AHI≥5, and 17 studies (89.5%) performed standard in-laboratory full-night polysomnography.

Table 1.

Characteristics of included study to evaluate the ethnicity and obesity in controls and patients with obstructive sleep apnea

Source	Country	No. of subjects	AHI	BMI (kg/m2)	NC	WC	WHR
Ip et al. [4]	China	30/30	1.8/35.7	26.5/27	38.5/38.9	89.9/91.9	0.9/0.91
Yucel et al. [5]	Turkey	24/47	2.1/29.6	34.1/32.9	37.4/39.3	NR	NR
Julia-Serda et al. [6]	Spain	92/115	3/46	29.7/32.6	40.6/43.5	102.5/111.4	NR
Zhao et al. [7]	China	36/32	5/32	23/26	38.1/40.3	NR	0.93/0.98
Sharma et al. [8]	India	80/40	1/32.2	25.3/29.8	36.3/39.3	92.7/106.9	0.85/1
Liu et al. [9]	China	18/58	5.7/37	25.6/28.4	37.6/40.1	NR	NR
Martinez-Rivera et al. [10]	Spain	68/124	4.9/38.9	31/31.4	40.5/42.5	100.7/106.3	0.94/0.98
Nakagawa et al. [11]	Japan	18/75	2.4/36	23.8/29.2	NR	86.7/97.7	NR
Jelic et al. [12]	USA	33/38	1/36	30/35	NR	96.5/109.2	0.88/0.93
Angelico et al. [13]	Italy	48/178	1.5/31.4	29.2/32.1	NR	101.2/110.1	0.94/0.97
Ugur et al. [14]	Turkey	24/73	2.6/18.7	29/32.4	42/42	99/101	NR
Kawaguchi et al. [15]	Japan	30/189	2.3/28.9	23.1/27.3	36.8/40.3	79.3/91.8	NR
Soylu et al. [16]	Turkey	68/431	2.3/36.5	26.9/32.2	37.7/43	96/113.1	NR
Lubrano et al. [17]	Italy	67/104	1.5/27.2	35.7/43.4	NR	111.5/129.2	0.96/0.99
Lin et al. [18]	China	45/113	3.3/23	22.4/22.7	NR	79.9/81.4	0.94/0.94
Apaydin et al. [19]	Turkey	20/67	2.4/47.6	27.4/31.6	30/41	98.5/109.7	0.94/0.99
Liu et al. [20]	China	19/130	3.2/30.9	24.4/28	36.2/39.9	NR	NR
Banhiran et al. [21]	Thailand	66/217	1.9/34.3	25.3/28.4	35.1/38.5	86.4/96	NR
Lim et al. [22]	Korea	17/102	1.9/36.9	25.6/26.6	37.7/40.3	89.9/96.1	0.9/0.95

Values are presented as control/obstructive sleep apnea.

AHI, apnea-hypopnea index; BMI, body mass index; NC, neck circumference; WC, waist circumference; WHR, waist-hip ratio; NR, not recorded.

BMI between Asians and Caucasians

There was a significant heterogeneity among all the studies (I2=74.2%, P<0.001), so we used a random effects model to derive the overall pooled estimates which showed that patients with OSA had greater BMI than controls (SMD, 0.62; 95% CI, 0.44 to 0.80; Fig. 2). However, funnel plots for BMI was asymmetric (Fig. 3) and Egger test suggested publication bias (P=0.007). Therefore, trim and fill analysis based on a random effects model was done to revise the publication bias. These results showed that BMI was no longer different between OSA patients and controls (SMD, 1.2; 95% CI, –0.40 to 2.79).

Fig. 2.

Forest plot for body mass index and neck circumference. OSA, obstructive sleep apnea; SMD, standard mean difference; CI, confidence inerval.

Fig. 3.

Funnel plot for body mass index, neck circumference, waist circumference, and waist-hip ratio. SE, standard error.

NC between Asians and Caucasians

There was significant heterogeneity among the studies (I2=81.0%, P<0.001), so we used a random effects model to derive the overall pooled estimates. This showed that patients with OSA had greater NC than control (SMD, 0.89; 95% CI, 0.63 to 1.14; Fig. 2). The funnel plot for NC was symmetrical (Fig. 3) and the Egger test for publication bias was also not significant (P=0.769). For subgroup analysis, we also used fixed effect model for Asians (I2=44.9%) and random effect model for Caucasians (I2=90.0%). Subgroup analysis also showed that patients with OSA had similar NC compared to controls for both Asians (SMD, 0.75; 95% CI, 0.53 to 0.98) and Caucasians (SMD, 0.939; 95% CI, 0.79 to 1.07). Heterogeneity between 2 subgroups was not significant (P=0.178), which means that the association between NC and OSA did not differ by the ethnicity.

WC between Asians and Caucasians

There was also significant heterogeneity among the studies (I2=67.4%, P<0.001). We used a random effects model to derive the overall pooled estimates. This showed that patients with OSA had greater WC than controls (SMD, 0.81; 95% CI, 0.64 to 0.99; Fig. 4). However, funnel plot for WC was asymmetric (Fig. 3) and Egger test suggested publication bias (P<0.001). Therefore, trim and fill analysis based on a random effects model was done to revise the publication bias and the results showed that WC was not different between OSA patients and controls (SMD, 3.97; 95% CI, –0.58 to 8.53).

Fig. 4.

Forest plot for waist circumference and waist-hip ratio. OSA, obstructive sleep apnea; SMD, standard mean difference; CI, confidence inerval.

WHR between Asians and Caucasians

There was significant heterogeneity among the studies (I2=79.5%, P<0.001) when WHR was examined. A random effects model was used to derive the overall pooled estimates which showed that patients with OSA had greater WHR than controls (SMD, 0.67; 95% CI, 0.39 to 0.96; Fig. 4). However, the funnel plot for WHR as asymmetric (Fig. 3) and Egger test suggested publication bias (P=0.018). Therefore, trim and fill analysis based on a random effects model was done to revise the publication bias. The result showed that WHR was no longer significant between OSA patients and controls (SMD, 0.01; 95% CI, –0.01 to 0.04).

DISCUSSION

There are various pathophysiologic factors associated with OSA such as obesity, unfavorable anatomical features, neuromuscular abnormalities, or genetic problems. Of these, obesity is thought to be one of the most common and important risk factors for OSA. Obesity is associated with increased narrowing and obstruction of the airway due to changes at the level of the soft palate, pharyngeal wall, and tongue base [28]. In addition, obesity may lead to modifications of neuromuscular function resulting in increased airway collapsibility and weakness of neuromuscular compensation [3,29].

The objective of this study was to identify any potential ethnicity-specific characteristics of obesity-related anthropometric measurements (BMI, NC, WC, and WHR). We performed a schematic meta-analysis by stratifying the raw data by ethnicity (Asian vs. Caucasian). The key findings of this study were that: (1) BMI, WC, and WHR were not different between patients with OSA and controls after evaluating for publication bias; (2) Patients with OSA had greater NC than controls in both Asians and Caucasians; and (3) Anthropometric phenotypes (BMI, NC, WC, and WHR) of Asian OSA patients were not different from Caucasian OSA patients. Our study results provide valuable data for clinicians when evaluating patients with different ethnicities with suspected OSA by identifying anthropometric characteristics associated with OSA that can be used to assist with the diagnosis prior to a standard polysomnography.

Previous studies have reported that obesity-related measurements such as BMI, WC, and WHR are significant risk factors for OSA [2,3]. Surprisingly, our meta-analysis found no difference in BMI, WC, and WHR between patients with OSA and controls when publication bias was considered. Only NC was greater and statistically significant among OSA patients regardless of the ethnicity. We used the ‘trim and fill method’ described by Duval and Tweedie [27] to identify and reconcile potential publication bias. This removes (trims) the smaller studies causing funnel plot asymmetry, and uses the trimmed funnel plot to estimate the true center of the funnel. Omitted studies and their missing counterparts are replaced around the center (fill). The results of this analysis are somewhat surprising, but raises the possibility that some of the anthropometric features of obesity, namely BMI, WC, and WHR, should be evaluated more carefully as true risk factors for OSA in future studies. Our findings may indicate that many non obese patients with OSA are still unrecognized or undiagnosed.

Among the four anthropometric measurements evaluated in our analysis, only NC was found to be statistically significant between OSA patients and controls. Although obesity is often regarded as a principle risk factor in the occurrence of OSA, our results suggest that the NC is more closely correlated with OSA without publication bias. Interestingly, we found no difference in NC between Asian and Caucasian patients with OSA and controls. Several studies report that Asians have more severe OSA than Caucasians. Ong et al. [30] evaluated demographic and polysomnographic data between Caucasians and Asians, and found that Asians with OSA had more severe disease than Caucasians when matched for age, gender, and BMI. And Li et al. [31] found that despite having a lower BMI than Caucasians, Asian men had more severe OSA. In this study, we investigated whether differences in obesity phenotypes between Asian and Caucasians were risk factors in OSA. However, we found that most of anthropometric data (BMI, WC, and WHR) lack of confidence due to publication bias and did not show interethnic differences. NC was the most reliable measurement and most likely represents a true risk factor for OSA in both Asian and Caucasian patients.

There are several limitations of this study. The trim and fill method was used to assess if publication bias was present and to estimate the effect when the bias is removed. It is one of the most common statistical methods used to resolve potential publication bias in meta-analysis, and the method has been found to be accurate [32]. However, it is not the only method available, and it requires several statistical assumptions, including that funnel plot asymmetry is caused solely by publication bias. Further studies are required to confirm our results. Finally, sex, age, and severity of OSA were not included in this meta-analysis due to lack of data in the reviewed studies.

In conclusion, obstructive sleep apnea is an airway disease known to be closely related to obesity. However, our study found that the many of the features of obesity such as BMT, WC, and WHR might not be significant risk factors for OSA due to publication bias. In this study, NC demonstrated a strong association with OSA, and this finding was not different between Asians and Caucasians.