Sadamu Kurono1,2, Yuka Kaneko1,2, Nariaki Matsuura3,4, Haruki Oishi1, Shinzaburo Noguchi5, Seung Jin Kim5, Yasuhiro Tamaki6, Takao Aikawa7, Yoshikazu Kotsuma8, Hideo Inaji6, Shuji Matsuura1. 1. Laboratory of Molecular Signature Analysis, Division of Health Sciences, Osaka University Graduate School of Medicine, Suita, Osaka, Japan. 2. Laboratory Chemicals Division, Wako Pure Chemical Industries, Ltd, Osaka, Japan. 3. Department of Molecular Pathology, Osaka University Graduate School of Medicine and Health Science, Suita, Osaka, Japan. 4. Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan. 5. Department of Breast and Endocrine Surgery, Osaka University Graduate School of Medicine, Suita, Osaka, Japan. 6. Department of Breast Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan. 7. Osaka Center for Cancer and Cardiovascular Diseases Prevention, Osaka, Japan. 8. Kotsuma Clinic, Osaka, Japan.
Abstract
PURPOSE: This research aimed to establish a diagnostic technique for breast cancer using nipple discharge (ND), with the objective of preventive diagnosis. ND has been proposed as a source of secreted proteomes that reflect early pathological changes in the ductal-lobular epithelial microenvironment, and could thus provide breast-specific cancer biomarkers that could be accessed noninvasively as a new clinical diagnostic technique. EXPERIMENTAL DESIGN: Minute amounts of ND from patients with and without breast cancer (n = 19 and 12, respectively) were collected at the hospital and kept frozen until just before use. They were analyzed using high-pH RP peptide fractionations/low-pH RP 2D nano-LC/ESI-MS/MS. Biomarker candidates were also investigated using Western blot analysis and sandwich ELISA on ND and/or sera. RESULTS: We found distinct tendencies in protein expression and three candidate breast cancer biomarkers (carbonic anhydrase 2, catalase, and peroxiredoxin-2) whose levels differed significantly between ND specimens from patients with and without breast cancer. CONCLUSIONS AND CLINICAL RELEVANCE: These tendencies in protein expression and markers provide new ways to identify breast cancer patients. Therefore, RP/RP 2D LC/MS/MS analyses of ND and the above three markers are supported as a new breast cancer diagnostic technique.
PURPOSE: This research aimed to establish a diagnostic technique for breast cancer using nipple discharge (ND), with the objective of preventive diagnosis. ND has been proposed as a source of secreted proteomes that reflect early pathological changes in the ductal-lobular epithelial microenvironment, and could thus provide breast-specific cancer biomarkers that could be accessed noninvasively as a new clinical diagnostic technique. EXPERIMENTAL DESIGN: Minute amounts of ND from patients with and without breast cancer (n = 19 and 12, respectively) were collected at the hospital and kept frozen until just before use. They were analyzed using high-pH RP peptide fractionations/low-pH RP 2D nano-LC/ESI-MS/MS. Biomarker candidates were also investigated using Western blot analysis and sandwich ELISA on ND and/or sera. RESULTS: We found distinct tendencies in protein expression and three candidate breast cancer biomarkers (carbonic anhydrase 2, catalase, and peroxiredoxin-2) whose levels differed significantly between ND specimens from patients with and without breast cancer. CONCLUSIONS AND CLINICAL RELEVANCE: These tendencies in protein expression and markers provide new ways to identify breast cancerpatients. Therefore, RP/RP 2D LC/MS/MS analyses of ND and the above three markers are supported as a new breast cancer diagnostic technique.
Authors: Bo Zhao; Jingjing Gao; Mahalia A C Serrano; Kathleen F Arcaro; S Thayumanavan; Richard W Vachet Journal: Anal Bioanal Chem Date: 2020-01-10 Impact factor: 4.142
Authors: Dan Shao; Lan Huang; Yan Wang; Xueteng Cui; Yufei Li; Yao Wang; Qin Ma; Wei Du; Juan Cui Journal: Database (Oxford) Date: 2021-10-13 Impact factor: 3.451