Literature DB >> 2694678

High level of expression of a protective antigen of schistosomes in Saccharomyces cerevisiae.

G Loison1, A Vidal, A Findeli, C Roitsch, J M Balloul, Y Lemoine.   

Abstract

Strains of Saccharomyces cerevisiae expressing P28-I, an antigen inducing protection against schistosomiasis, have been constructed. Transformants containing a very high copy number of a P28-I expression vector were selected by genetic complementation involving deficient LEU2 or URA3 alleles carried by plasmids. Using the ura3 fur1 auto-selection system, constitutive and stable expression of P28-I could be obtained in cultures grown in rich medium. The accumulation of the foreign protein exceeds 25% of total yeast proteins when estimated by Coomassie Brilliant Blue staining of SDS-PAGE. Moreover, P28-I which was located intracellularly was soluble and biologically active.

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Year:  1989        PMID： 2694678     DOI： 10.1002/yea.320050609

Source DB:  PubMed          Journal:  Yeast        ISSN： 0749-503X            Impact factor:   3.239


  9 in total

1.  Direct plasmid shuttling from yeast into Escherichia coli by electroduction.

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Journal:  Nucleic Acids Res       Date:  1994-04-25       Impact factor: 16.971

2.  Quantifying Nucleation In Vivo Reveals the Physical Basis of Prion-like Phase Behavior.

Authors:  Tarique Khan; Tejbir S Kandola; Jianzheng Wu; Shriram Venkatesan; Ellen Ketter; Jeffrey J Lange; Alejandro Rodríguez Gama; Andrew Box; Jay R Unruh; Malcolm Cook; Randal Halfmann
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3.  Recombinant outer-surface protein A (des-Cys1-OspA) from the Lyme disease spirochete Borrelia burgdorferi: high production levels in Saccharomyces cerevisiae yeast cultures.

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Journal:  Appl Microbiol Biotechnol       Date:  1996-01       Impact factor: 4.813

Review 4.  An Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae.

Authors:  Verena Siewers
Journal:  Methods Mol Biol       Date:  2022

5.  Stabilization of methionine-rich protein in Saccharomyces cerevisiae: targeting of BZN protein into the peroxisome.

Authors:  J M Nicaud; A Raynal; A Beyou; M Merkamm; H Ito; N Labat
Journal:  Curr Genet       Date:  1994 Nov-Dec       Impact factor: 3.886

6.  Estradiol-inducible squelching and cell growth arrest by a chimeric VP16-estrogen receptor expressed in Saccharomyces cerevisiae: suppression by an allele of PDR1.

Authors:  D M Gilbert; D M Heery; R Losson; P Chambon; Y Lemoine
Journal:  Mol Cell Biol       Date:  1993-01       Impact factor: 4.272

7.  Homo- and heterodimers of the retinoid X receptor (RXR) activated transcription in yeast.

Authors:  D M Heery; B Pierrat; H Gronemeyer; P Chambon; R Losson
Journal:  Nucleic Acids Res       Date:  1994-03-11       Impact factor: 16.971

8.  Auxin-mediated induction of GAL promoters by conditional degradation of Mig1p improves sesquiterpene production in Saccharomyces cerevisiae with engineered acetyl-CoA synthesis.

Authors:  Irfan Farabi Hayat; Manuel Plan; Birgitta E Ebert; Geoff Dumsday; Claudia E Vickers; Bingyin Peng
Journal:  Microb Biotechnol       Date:  2021-09-09       Impact factor: 5.813

9.  EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple Genomic Integrations in Saccharomyces cerevisiae.

Authors:  Jérôme Maury; Susanne M Germann; Simo Abdessamad Baallal Jacobsen; Niels B Jensen; Kanchana R Kildegaard; Markus J Herrgård; Konstantin Schneider; Anna Koza; Jochen Forster; Jens Nielsen; Irina Borodina
Journal:  PLoS One       Date:  2016-03-02       Impact factor: 3.240
  9 in total

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