Literature DB >> 35781196

An Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae.

Verena Siewers1.   

Abstract

For genetic manipulation of yeast, numerous selection marker genes have been employed. These include prototrophic markers, markers conferring drug resistance, autoselection markers, and counterselectable markers. This chapter describes the different classes of selection markers and provides a number of examples for different applications.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Autoselection; Auxotrophy; Counterselection; Drug resistance; Marker loop-out

Mesh:

Substances:

Year:  2022        PMID: 35781196     DOI: 10.1007/978-1-0716-2399-2_1

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  62 in total

1.  Disintegrator vectors for single-copy yeast chromosomal integration.

Authors:  Ivan Sadowski; Ting-Cheng Su; Jennifer Parent
Journal:  Yeast       Date:  2007-05       Impact factor: 3.239

2.  Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications.

Authors:  C B Brachmann; A Davies; G J Cost; E Caputo; J Li; P Hieter; J D Boeke
Journal:  Yeast       Date:  1998-01-30       Impact factor: 3.239

3.  A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast.

Authors:  U Gueldener; J Heinisch; G J Koehler; D Voss; J H Hegemann
Journal:  Nucleic Acids Res       Date:  2002-03-15       Impact factor: 16.971

4.  A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15.

Authors:  G J Cost; J D Boeke
Journal:  Yeast       Date:  1996-08       Impact factor: 3.239

5.  A method for performing precise alterations in the yeast genome using a recycable selectable marker.

Authors:  F Längle-Rouault; E Jacobs
Journal:  Nucleic Acids Res       Date:  1995-08-11       Impact factor: 16.971

6.  Heterologous URA3MX cassettes for gene replacement in Saccharomyces cerevisiae.

Authors:  A L Goldstein; X Pan; J H McCusker
Journal:  Yeast       Date:  1999-04       Impact factor: 3.239

7.  Molecular cloning and application of a gene complementing pantothenic acid auxotrophy of sake yeast Kyokai no. 7.

Authors:  H Shimoi; M Okuda; K Ito
Journal:  J Biosci Bioeng       Date:  2000       Impact factor: 2.894

8.  Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiae.

Authors:  Sayoko Ito-Harashima; John H McCusker
Journal:  Yeast       Date:  2004-01-15       Impact factor: 3.239

9.  Production of a thermostable alcohol dehydrogenase from Rhodococcus ruber in three different yeast species using the Xplor®2 transformation/expression platform.

Authors:  Martin Giersberg; Adelheid Degelmann; Rüdiger Bode; Michael Piontek; Gotthard Kunze
Journal:  J Ind Microbiol Biotechnol       Date:  2012-05-15       Impact factor: 3.346

10.  A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

Authors:  R S Sikorski; P Hieter
Journal:  Genetics       Date:  1989-05       Impact factor: 4.562

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