| Literature DB >> 26944455 |
Nastasia Soanen1, Elise Da Silva1, Christine Gardarin1, Philippe Michaud1, Céline Laroche2.
Abstract
With the aim to optimize the production of exopolysaccharide (EPS) by Porphyridium marinum, cultures in photobioreactors were conducted on a modified Provasoli medium (P) and compared to a new medium (Pm) with an elemental composition of N0.0205S0.0597P0.005. Cultivation on this medium allowed the increase of EPS concentration up to 2.5gL(-1), without modification of the EPS productivity (0.096gL(-1)) and EPS structure. In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production. A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.Entities:
Keywords: Microalgae; Photobioreactor; Polysaccharide; Porphyridium; Semi-continuous culture
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Year: 2016 PMID： 26944455 DOI： 10.1016/j.biortech.2016.02.075
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642