| Literature DB >> 26938994 |
Shao Su1, Haofan Sun1, Wenfang Cao1, Jie Chao1, Hongzhen Peng2, Xiaolei Zuo2, Lihui Yuwen1, Chunhai Fan1,2, Lianhui Wang1.
Abstract
A MoS2-based electrochemical aptasensor has been developed for the simultaneous detection of thrombin and adenosine triphosphate (ATP) based on gold nanoparticles-decorated MoS2 (AuNPs-MoS2) nanocomposites. Two different aptamer probes labeled with redox tags were simultaneously immobilized on an AuNPs-MoS2 film modified electrode via Au-S bonds. The aptamers presented structural switches with the addition of target molecules (thrombin and ATP), resulting in methylene blue (MB) far from or ferrocene (Fc) close to the electrode surface. Therefore, a dual signaling detection strategy was developed, which featured both "signal-on" and "signal-off" elements in the detection system because of the target-induced structure switching. This proposed aptasensor could simultaneously determine ATP and thrombin as low as 0.74 nM ATP and 0.0012 nM thrombin with high selectivity, respectively. In addition, thrombin and ATP could act as inputs to activate an AND logic gate.Entities:
Keywords: aptasensor; gold nanoparticles; logic gates; molybdenum disulfide; simultaneous detection
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Year: 2016 PMID： 26938994 DOI： 10.1021/acsami.5b12833
Source DB: PubMed Journal: ACS Appl Mater Interfaces ISSN： 1944-8244 Impact factor: 9.229