Literature DB >> 26938491

A highly sensitive aptasensor for OTA detection based on hybridization chain reaction and fluorescent perylene probe.

Bin Wang1, Yuanya Wu2, Yanfen Chen2, Bo Weng3, Liqun Xu2, Changming Li2.   

Abstract

An optical aptasensor was developed for ultrasensitive detection of ochratoxin A (OTA) based on hybridization chain reaction (HCR) amplification strategy and fluorescent perylene probe (PAPDI)/DNA composites. Dendritic DNA concatamers were synthesized by HCR strategy and modified on magnetic nanoparticles through aptamer as medium. A large amount of PAPDI probe aggregated under the induction of DNA concatamers and caused fluorescence quenching. In the presence of OTA, the PAPDI/DNA composites were released from magnetic nanoparticles due to the strong affinity between aptamer and OTA. In ethanol, PAPDI monomers disaggregated and produced strong fluorescence. The present method displays excellent sensitivity and selectivity towards OTA.
Copyright © 2016 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Aptamer; Hybridization chain reaction; OTA; Optical aptasensor; Perylene probe

Mesh：

Substances：

Year:  2016        PMID： 26938491     DOI： 10.1016/j.bios.2016.02.062

Source DB:  PubMed          Journal:  Biosens Bioelectron        ISSN： 0956-5663            Impact factor:   10.618


  8 in total

1.  A fluorometric aptamer-based assay for ochratoxin A using magnetic separation and a cationic conjugated fluorescent polymer.

Authors:  Yufei Liu; Huijuan Yan; Jingfang Shangguan; Xue Yang; Meili Wang; Wei Liu
Journal:  Mikrochim Acta       Date:  2018-08-22       Impact factor: 5.833

2.  Fluorometric aptamer based assay for ochratoxin A based on the use of exonuclease III.

Authors:  Renjie Liu; Hua Wu; Lei Lv; Xiaojiao Kang; Chengbi Cui; Jin Feng; Zhijun Guo
Journal:  Mikrochim Acta       Date:  2018-04-14       Impact factor: 5.833

Review 3.  A review on recent developments in optical and electrochemical aptamer-based assays for mycotoxins using advanced nanomaterials.

Authors:  K Yugender Goud; K Koteshwara Reddy; M Satyanarayana; Shekher Kummari; K Vengatajalabathy Gobi
Journal:  Mikrochim Acta       Date:  2019-12-07       Impact factor: 5.833

4.  A switchable and signal-amplified aptasensor based on metal organic frameworks as the quencher for turn-on detection of T-2 mycotoxin.

Authors:  Xinliu Tan; Weidao Yu; Yuwen Wang; Ping Song; Qing Xu; Dengming Ming; Yaqiong Yang
Journal:  Anal Bioanal Chem       Date:  2021-08-24       Impact factor: 4.142

Review 5.  Nanomaterial-based aptamer biosensors for ochratoxin A detection: a review.

Authors:  Xiujin Chen; Dong Gao; Zhaozhou Li; Yao Wang; Fengxia Sun; Caixia Qiu; Kaifeng He; Jing Wang
Journal:  Anal Bioanal Chem       Date:  2022-03-16       Impact factor: 4.142

6.  A Label-Free Aptasensor for Ochratoxin a Detection Based on the Structure Switch of Aptamer.

Authors:  Feng Liu; Ailing Ding; Jiushang Zheng; Jiucun Chen; Bin Wang
Journal:  Sensors (Basel)       Date:  2018-06-01       Impact factor: 3.576

7.  A Novel Fluorescent Aptasensor Based on Real-Time Fluorescence and Strand Displacement Amplification for the Detection of Ochratoxin A.

Authors:  Wei Guo; Haoyu Yang; Yunzhe Zhang; Hao Wu; Xin Lu; Jianxin Tan; Wei Zhang
Journal:  Foods       Date:  2022-08-13

8.  Label-Free G-Quadruplex Aptamer Fluorescence Assay for Ochratoxin A Using a Thioflavin T Probe.

Authors:  Kefeng Wu; Changbei Ma; Han Zhao; Hailun He; Hanchun Chen
Journal:  Toxins (Basel)       Date:  2018-05-12       Impact factor: 4.546
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.