| Literature DB >> 2693426 |
Abstract
A membrane filter-Endo agar method for enumerating Escherichia coli as distinct from other coliforms in drinking water was developed. Membranes containing coliform colonies are transferred to nutrient agar containing 4-methyl umbelliferyl-beta-D-glucuronide (MUG) and incubated at 35 degrees C for 4 h. The MUG is hydrolyzed by the glucuronidase of E. coli and the fluorogenic product is visualized. The method recovered 98% of E. coli without false positives and is proposed as an additional test in routine water examination for the detection of pollution.Entities:
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Year: 1989 PMID： 2693426 DOI： 10.1111/j.1365-2672.1989.tb02503.x
Source DB: PubMed Journal: J Appl Bacteriol ISSN： 0021-8847