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Literature DB >> 26924570

Multicolor Immunofluorescent Imaging of Complex Cellular Mixtures on Micropallet Arrays Enables the Identification of Single Cells of Defined Phenotype.

Trisha M Westerhof¹, Guann-Pyng Li², Mark Bachman³, Edward L Nelson^1,4.

Abstract

A Micropallet-Array-based strategy allowing the identification of cells of defined phenotype in complex mixtures, such as would be obtained from a tissue biopsy, is presented. Following the distribution of single adherent cells from the mixture on individual pedestals, termed "micropallets", immunofluorescent confocal imaging is applied to interrogate the expression of five cell surface molecules.

Entities: CellLine Chemical Disease Gene Species

Keywords: biomems; immunofluorescent confocal microscopy; micropallet array; single cell analysis

Mesh：

Substances：
Fluorescent Dyes

Year: 2016 PMID： 26924570 PMCID： PMC5629097 DOI： 10.1002/adhm.201500859

Source DB: PubMed Journal: Adv Healthc Mater ISSN： 2192-2640 Impact factor: 9.933

20 in total

1. Fabrication and biological evaluation of uniform extracellular matrix coatings on discontinuous photolithography generated micropallet arrays.

Authors: Nicholas M Gunn; Mark Bachman; Guann-Pyng Li; Edward L Nelson
Journal: J Biomed Mater Res A Date: 2010-11 Impact factor: 4.396

2. Micropallet arrays for the separation of single, adherent cells.

Authors: Georgina To'a Salazar; Yuli Wang; Grace Young; Mark Bachman; Christopher E Sims; G P Li; Nancy L Allbritton
Journal: Anal Chem Date: 2007-01-15 Impact factor: 6.986

3. Characterization of the laser-based release of micropallets from arrays.

Authors: Georgina To'a Salazar; Yuli Wang; Christopher E Sims; Mark Bachman; G P Li; Nancy L Allbritton
Journal: J Biomed Opt Date: 2008 May-Jun Impact factor: 3.170

Review 4. The role of stem and circulating cells in cancer metastasis.

Authors: Ruby Ghadially
Journal: J Surg Oncol Date: 2011-05-01 Impact factor: 3.454

5. To what extent are the "sets" of biologic properties expressed by hypoxic cancer cells and by cancer "stem" cells equivalent, intersecting, disjoint or complementary? Some implications for the design of cancer therapies.

Authors: K M Anderson; P Tsui; P Guinan; M Rubenstein
Journal: Med Hypotheses Date: 2006-08-28 Impact factor: 1.538

Multicolor Immunofluorescent Imaging of Complex Cellular Mixtures on Micropallet Arrays Enables the Identification of Single Cells of Defined Phenotype.

1. Fabrication and biological evaluation of uniform extracellular matrix coatings on discontinuous photolithography generated micropallet arrays.

2. Micropallet arrays for the separation of single, adherent cells.

3. Characterization of the laser-based release of micropallets from arrays.

Review 4. The role of stem and circulating cells in cancer metastasis.

5. To what extent are the "sets" of biologic properties expressed by hypoxic cancer cells and by cancer "stem" cells equivalent, intersecting, disjoint or complementary? Some implications for the design of cancer therapies.

Review 6. The role of circulating endothelial progenitor cells in tumor angiogenesis.

Review 7. Cancer related inflammation: the macrophage connection.

8. Photoresist with low fluorescence for bioanalytical applications.

Review 9. Endothelial progenitor cell biology in disease and tissue regeneration.

Review 10. Cancer stem cells: implications for the progression and treatment of metastatic disease.

1. Highly efficient cellular cloning using Ferro-core Micropallet Arrays.