A pilot study on faecal MMP-9: a new noninvasive diagnostic marker of colorectal cancer.

BACKGROUND: Colorectal cancer (CRC) is one of the leading malignancies worldwide, therefore cheap noninvasive screening methods are of great importance. Matrix-metalloproteinase-9 (MMP-9) has a role in the progression of CRC, and its level is elevated in tumour biopsies. Faecal MMP-9 levels are increased in active ulcerative colitis patients, but in CRC patients, they have never been measured. We aimed to assess the faecal MMP-9 levels in patients undergoing total colonoscopy according to endoscopic and histological diagnosis.
METHODS: One hundred and nine patients provided faecal samples for MMP-9 analysis. A total colonoscopy was performed; suspicious lesions were evaluated by histology. Faecal MMP-9 levels were measured by ELISA.
RESULTS: The number of patients allocated to different groups were: negative/diverticulosis: 34 (referred to as controls); hyperplastic polyps: 15; adenomas: 32 (22 at high risk); and CRC: 28. Faecal MMP-9 was significantly increased in CRC compared with all other groups (P<0.001). Faecal MMP-9 was suitable to distinguish CRC patients from controls (sensitivity: 89.3%; specificity: 91.2%). By means of a lower cutoff level, faecal MMP-9 identified high-risk adenomas besides CRC (sensitivity: 76%; specificity: 85.3%). This lower cutoff level screened 59% of high-risk adenomas.
CONCLUSIONS: Faecal MMP-9 may be a promising new noninvasive marker in CRC.

Colorectal cancer (CRC) represents a major cause of morbidity and mortality in Western societies, and the incidence in developing countries is also rapidly growing (Sung ; Siegel ). In Europe, CRC is the second most frequent type of malignancy among males and females, with an estimated incidence of >400 000 new cases per year (Ferlay ). The high frequency of the disease and the fact that the prognosis correlates well with the stage at diagnosis makes CRC screening extremely important. Colonoscopy is the gold standard for CRC screening by its high sensitivity and specificity but has also disadvantages, such as risk of complications, high costs and lower acceptance by patients as a screening method. Therefore, noninvasive methods are required to identify patients at high risk of CRC where colonoscopy needs to be performed.

CRC screening recommendations vary between countries but mostly consist of an annual or biannual faecal occult blood test (FOBT) combined with colonoscopy in case of positivity or a colonoscopy every 10 years, in the population aged >50 years (Binefa ). Two basic types of FOBT exist, based on the imperceptible intermittent leakage of blood from CRC and high-risk adenomas in stool (Binefa ). The guaiac test (gFOBT) detects the peroxidase activity in the haemoglobin haeme subunit, therefore it is nonspecific for human haemoglobin and theoretically requires dietary restrictions a few days before performing the test, but large studies have found no significant advantage of the dietary modifications, therefore lately they are not recommended by many authors (Konrad, 2010). A minimum of two faecal samples are necessary from different stools to obtain an appropriate sensitivity. Sensitivity for CRC ranges from 6.2% to 83.3%, depending on the test used, while specificity is >80% (Binefa ). The immunochemical faecal occult blood test (FIT) is based on specific monoclonal or polyclonal antibodies against human haemoglobin, thus it does not require dietary alterations. Contrary to gFOTB, the analysis by FIT can be automated and its threshold value is adjustable by the user. According to the literature, the sensitivity of FIT is extremely variable, from 5.4% to nearly 98%, depending on the test used (Binefa ). Specificity can range from 77% to 99%. The European Guidelines for quality assurance of CRC recommends the use of FIT rather than gFOBT (Halloran ). However, reliable tests with a less variable sensitivity and specificity are still lacking.

Matrix-metalloproteinases (MMPs) are a family of Zn2+-containing endopeptidases, secreted by various cell types, such as tumour cells, mesenchymal cells, fibroblasts and inflammatory cells, such as monocytes, lymphocytes and neutrophils. Increased expression of MMP-9 has been detected in tissue samples from rectal carcinoma and colon cancer, where it correlates with poor prognosis (Unsal ; Araujo ; Yang ). Recently, we have shown that faecal MMP-9 levels are elevated in active ulcerative colitis (UC) patients compared with patients with a functional gastrointestinal disorder (diarrhoea-predominant irritable bowel syndrome) or healthy controls (Annahazi ). Faecal MMP-9 levels were found to be a predictor of disease activity in UC patients (Annahazi ), and had a high specificity in the diagnosis of pouchitis (Farkas ). However, faecal MMP-9 levels in CRC patients have not been investigated.

Based on this background, we aimed to measure faecal MMP-9 levels in patients undergoing colonoscopic examination in our hospital and to analyse the results according to the different colonoscopic and histological findings.

Materials and methods

Ethical considerations

The study protocol was approved by the Ethical Committee of the University of Szeged, Szeged, Hungary. All subjects provided written and informed consent to participate.

Patient selection and sample collection

Overall, 109 patients (male/female: 58/51, age: 29–91 years, mean: 66.0 years) referred to our specialist colorectal unit in the First Department of Medicine, Szeged, Hungary were enrolled in the study, who all underwent colonoscopy. No patients had a history of UC or a history of other cancers. One patient, who was later identified as a rectal adenocarcinoma patient, had liver metastases. Faecal samples were collected from all patients 2–4 days before the colonoscopy and were frozen to −20 °C within 1 h after defaecation. All colonoscopies were completed until reaching the terminal ileum. During colonoscopy, biopsies were taken from all suspect lesions, and histology was performed. Based on the colonoscopic and histological results, patients were allocated to five groups: negative, diverticulosis, hyperplastic polyp, adenoma, and CRC. Adenomas were considered at increased risk of malignisation when one of the followings was fulfilled: polyp size >10 mm; villous adenoma or tubulovillous adenoma with at least 20% villous component; high-grade dysplasia; or multiple adenoma (>3).

Validation assay

Our group was the first to use the commercial Human Quantitative Enzyme-Linked Immunosorbent Assay Kit from R&D Systems (Abingdon, UK, Cat. No. DMP900) for the measurement of MMP-9 levels in faeces. Therefore, we have performed a validation assay to test whether the faecal matrix itself has an influence on MMP-9 determination and whether the kit from R&D Systems is able to determine MMP-9 from human faecal extracts.

Active human MMP-9 full-length protein (Abcam LTD, Cambridge, UK; Cat. No.: ab157344; LOT No: GR218764-1) was added to the extraction medium. Control faecal samples were weighed and 1 ml extraction medium with or without added MMP-9 was given to 0.25 mg sample (the sample to extraction medium ratio was 1 : 4). The samples were immediately homogenised for 2 × 30 s and incubated for 10 min at room temperature. Subsequently, all homogenates were centrifuged at 1500 g for 10 min followed by a second centrifugation of the supernatants from the first centrifugation step at 10 000 g for 10 min. The final supernatants were processed for linearity (parallelism) test. The extract containing added MMP-9 was serially diluted 1+1 to get 50, 25, 12.5 and 6.25% of the original MMP-9 content with the extract of the same faeces containing no added MMP-9. Then all samples, including 100% of added MMP-9 extract and the native control extract without added MMP-9, were subjected to MMP-9 measurement by the above-mentioned ELISA kit for human MMP-9. The kit is able to detect the human 92 kDa Pro-MMP-9 and 82 kDa active form of MMP-9 as stated by the manufacturer. The added MMP-9 protein obtained for this evaluation was the 92 kDa Pro-MMP-9.

Measurement of faecal MMP-9

Samples were stored on −20 °C for a maximum of 3 months and then thawed at 4 °C for 1 day. In all, 1.0 g of each faecal sample was diluted, mixed and homogenised in 4 ml of ice-cold Tris-buffer (0.15 M NaCl+20 mM Tris-HCl, pH:8.3). After centrifugation (10 min, 4500 r.p.m., 4 °C), pellets were discarded and supernatants were recentrifuged (10 min, 10 000 g; 4 °C). The final supernatants were filtered by 0.8-μm pore-sized syringe filters, and the aliquots were stored at −20 °C until analysed. MMP-9 was measured by the quantitative enzyme-linked immunosorbent assay described above.

Statistical analysis

Continuous data were presented as mean±s.d. Kruskal–Wallis test was used to compare faecal MMP-9 levels of the different groups (control, hyperplastic polyp, adenoma and CRC). MMP-9 levels of high- and low-risk adenoma subgroups were analysed by the Mann–Whitney test. The different subgroups according to tumour localisation or stage were compared with the Kruskal–Wallis test. Differences were considered statistically significant when P<0.05.

Receiver operating characteristic (ROC) curve analysis was performed to determine the predictive power of the MMP-9 level. Area under ROC curve (AUC), confidence interval for AUC, sensitivity and specificity were calculated. The optimal cutoff values were determined using Youden's index, if the AUC was >0.8 (Baker and Kramer, 2007) The cutoff level was determined by the maximum value of Youden's index (sensitivity+specificity−1). Statistical analyses were performed by the R (version 3.1.2) statistical software (https://www.r-project.org/).

Results

The concentrations measured in ng ml−1 are shown in Figure 1. The figure clearly shows a linear concentration change corresponding to dilution. It indicates that (i) the faecal matrix itself has no influence on MMP-9 determination; and (ii) the kit from R&D Systems is able to determine MMP-9 from human faecal extracts.

Figure 1

MMP-9 concentration levels at different dilutions in the validation assay.

Faecal MMP-9 levels between groups

Seventeen patients (male/female: 8/9; mean age: 59.7 years) were found negative on total colonoscopy and 17 patients had no other finding from noninflamed diverticulosis (male/female: 3/14; mean age: 71.8 years). There was no significant difference between these two groups in terms of faecal MMP-9 levels (P=0.49), therefore these 34 patients were used as polyp-free/non-malignant controls (mean age: 65.7 years). Out of the other patients, 15 had hyperplastic polyps (male/female: 9/6; mean age: 62.3 years), 32 had adenomas (male/female: 21/11; mean age: 67.0) and 28 had CRC (all adenocarcinomas; male/female: 17/11; mean age: 67.2 years) according to the endoscopic and histological examinations. There were no statistically significant differences between the four groups (controls, hyperplastic polyps, adenomas, CRC) regarding the age. Faecal MMP-9 levels were significantly different between the four groups (Figure 2, P<0.0001). Faecal samples from control patients had very low MMP-9 levels (mean: 0.118±0.057 ng ml−1). Patients with hyperplastic polyps also presented with low faecal MMP-9 levels (mean: 0.112±0.073 ng ml−1). Adenoma patients had slightly, but not significantly elevated faecal MMP-9 levels (mean: 0.656±0.396 ng ml−1). Nevertheless, patients with CRC had a significantly increased mean of faecal MMP-9 levels (mean: 7.028±3.722 ng ml−1). To distinguish CRC patients from controls, the cutoff level was determined at 0.23 ng ml−1 (AUC=0.913; 95% CI: 0.833–0.994; P<0.001). This cutoff level was associated with a sensitivity of 89.3% and a specificity of 91.2% (Figure 3). Patients with hyperplastic polyps (AUC=0.526; 95% CI: 0.340–0.711; P=0.779) were not distinguishable from control individuals by ROC analysis. Among adenomas, 22 out of 32 were at increased risk of malignancy, with a mean faecal MMP-9 level significantly elevated compared with low-risk adenomas (mean of high-risk adenomas: 0.936±0.570 vs mean of low-risk adenomas: 0.040±0.017; P<0.05; Figure 4). Low-risk adenomas could not be distinguished from controls by faecal MMP-9 levels (AUC=0.456; 95% CI: 0.236–0.676; P=0.706) When ROC analysis was performed with an aim to select high-risk adenomas and CRC from controls, the cutoff level was determined at 0.085 ng ml−1. In this case, sensitivity was 76% and specificity was 85.3% (AUC=0.806; 95% CI: 0.710–0.902; P<0.001). With this lower cutoff level, 59% of high-risk adenomas were identified. By ROC analysis of high-risk adenomas vs controls, the cutoff level would be similarly 0.085 ng ml−1 (AUC=0.670; 95% CI: 0.507–0.833; P=0.033).

Figure 2

Faecal MMP-9 levels of the different groups (based on colonoscopy and histology). Abbreviation: CRC=colorectal carcinoma.

Figure 3

ROC curve of CRC

Figure 4

Faecal MMP-9 levels in adenomas.

Faecal MMP-9 levels according to CRC localisation and stage

The number of patients regarding the site of the CRC was as follows: rectum: 14, sigmoid colon: 6, lineal flexure: 1, transverse colon: 2, hepatic flexure: 2, ascending colon: 1, and coecum: 2 patients. If faecal MMP-9 levels are analysed according to the site of the CRC, no significant difference was observed (P=0.146), although a trend was seen towards higher levels in case of a rectal tumour (mean: 12.33±7.274 ng ml−1) compared with left-sided colon (lineal flexure–sigmoid colon; mean: 1.619±0.978 ng ml−1) and transverse-right-sided colon (from coecum to transverse colon; mean: 1.830±0.656 ng ml−1; Figure 5A). According to Dukes' classification, no significant difference could be seen between the groups (Figure 5B; P=0.236).

Figure 5

Faecal MMP-9 levels in CRC patients. Values according to (A) localisation and (B) Dukes' stages of CRC.

Discussion

To the best of our knowledge, we report for the first time that MMP-9 levels are elevated in faecal specimens from CRC patients compared with patients without polypoid lesions. Furthermore, faecal MMP-9 can be used to distinguish CRC patients from patients with no polypoid lesions, with a high sensitivity and specificity. Additionally, with a lower cutoff level, faecal MMP-9 is able to identify nearly 60% of patients with a high-risk adenoma.

MMP-9 is a gelatinase capable of the degradation of the extracellular matrix, which is believed to have a role in the progression and metastasis formation of many tumours, including colon cancer (Yang ). Apart from colon cancer cells, tumour-infiltrating neutrophils are also an important source of MMP-9, as a major angiogenesis-inducing factor (Hyuga ; Deryugina ). Serum MMP-9 was suggested as an early noninvasive biomarker for breast cancer (Provatopoulou ) and a prognostic marker for lung cancer (Ylisirnio ). Urinary MMPs were found elevated not only in bladder, renal and prostate cancers but also in anatomically distant tumours, including breast, brain, and hematological malignancies (Moses ; Chan ; Fernandez ; Smith ). Recently, significant efforts have been made to explore the potential role of MMP-9 as a biomarker in CRC. In the serum, it has been suggested as a biomarker of CRC in symptomatic patients (Hurst ), and its levels correlated with the Dukes' stage (Mroczko ). Furthermore, it has been recently shown that serum levels of neutrophil gelatinase-associated lipocalin in complex with MMP-9 are elevated in CRC patients, but it was found unsuitable as a diagnostic marker, as its discriminative power was very poor (Duvillard ). We have found the discriminative power of faecal MMP-9 levels in CRC patients sufficient, which can be explained by the fact that faecal MMP-9 could be more informative for gastrointestinal malignancies than urine or serum. Our method does not require a preceding diet, and one faecal sample gave a sufficient sensitivity for discrimination. Its analysis is automated, and the cut of concentration is adjustable. In terms of these characteristics, faecal MMP-9 measurement, similarly to FIT, is more convenient for clinical use than gFOBT. Furthermore, faecal MMP-9 measurement provides a higher sensitivity and specificity for CRC than gFOBT and than many types of FIT.

In the past decade, numerous faecal molecular markers have been described, which are based on the detection of genetic mutations and epigenetic alterations, linked to the carcinogenetic process (for a review, see Kanthan ). Genetic markers are claimed to be more sensitive than FOBTs (Kanthan ). However, one disadvantage of these markers is that they are not present in every colorectal carcinoma. To overcome this problem, genetic markers are often combined in a panel, but this may lead to a decrease in specificity (Kanthan ). Furthermore, an important limitation of these methods is their high costs. Current evidence is not sufficient to accept the routine use of faecal genetic markers, and the European Guidelines for quality assurance have stated that they are currently unsuitable for CRC screening (Halloran ). Similarly to molecular tests, our method also has the advantage over FOBTs in that it detects a marker originating directly from cancer, which, contrary to bleeding, is supposed to be permanent. However, the costs of faecal MMP-9 measurement are far beyond those of molecular tests, as they are <10 USD per sample, which is comparable to the costs of FOBT. This method is markedly cheaper than faecal DNA tests, which cost around 350 USD per sample (Lansdorp-Vogelaar ).

The process of transformation from normal epithelium to cancer takes 10–15 years in most cases, leaving a possibility to remove precancerous lesions before they turn to definitive cancer (Binefa ). Therefore, a reliable marker detecting high-risk adenomas is of high importance. The sensitivity of FIT for advanced adenomas is only around 20–33% (Morikawa ; Haug ). DNA tests are characterised by a much higher sensitivity, for example, K-ras mutations are detected in the faeces of around 50% of individuals with adenomas >1 cm (Mak ), p53 mutations in up to 64% of severely dysplastic polyps (Tagore ) and 4–26% of adenomas (Mak ). Methods based on the detection of epigenetic changes appear to have a wide range of sensitivity, for example, the methylation of SFRP2 can be detected in the faeces of 52.4% of individuals with adenomas (Huang ), while the methylation of methylguanine DNA methyltransferase can be found in 36% of the faeces of adenoma patients but that of the human mut I homolog-1 in only 11% (Baek ). Faecal tumour M2 pyruvate kinase, a promising marker in CRC screening, showed a sensitivity of 22% for advanced adenomas (Haug ). Serum MMP-9 levels were elevated not only in cancer but also in colorectal adenoma patients compared with healthy subjects (Mroczko ). Similarly, MMP-9 expression was found increased not only in tissue samples from CRC but also in those from highly dysplastic adenomatous polyps compared with hyperplastic polyps or adenomas with low dysplasia (Odabasi ). Furthermore, MMP-9 protein expression has been elevated in adenomas with high grade dysplasia compared with other adenomas or normal colonic tissue (Herszenyi ). Our results have shown that faecal MMP-9 levels from high-risk adenoma patients are significantly elevated compared with patients with low-risk adenomas. However, with a lower cutoff level, our marker identified 59% of high-risk adenoma patients as positive. This result is definitely higher than the usual sensitivity of FOBTs and comparable to that of molecular tests but without the high costs of the latter.

We did not find any significant difference between the faecal MMP-9 levels of the groups with different tumour location. Nevertheless, a tendency could be seen towards the highest faecal MMP-9 levels in rectal adenocarcinoma patients. This is probably not related to the nature of rectal tumours but could be explained with a less time for degradation of the marker in the intestinal lumen, similarly to that seen by FIT (Morikawa ).

Our study has some limitations. As our department is a specific colorectal center, our patients were a selected population not representing the general population. Patients were on a low-fibre diet at the time of faecal sample collection, as a preparation for the upcoming colonoscopy. Data on the time of the day at faecal sample production was not collected in our study. Although the percentage of cancer and adenoma found on colonoscopy was relatively high, the number of patients in the different subgroups (i.e., tumour localisation, Dukes' stage) may be insufficient for the detection of minor differences. Moreover, MMP-9 is also elevated in gut inflammation, which may limit its use in case of, for example, IBD. We hypothesise that, in a more general population, elevated faecal MMP-9 levels in some cases could be related to inflammation or other cancers in the gastrointestinal tract. However, even in this case, the method would be a good predictor of the presence of a nonfunctional gastrointestinal disorder, based on our previous results showing that faecal MMP-9 measurement can well discriminate IBS-D patients and healthy subjects from UC patients. A further limitation is that, owing to the different cutoff levels calculated on the same sample groups, the performance of the method may be overestimated. A further study with a larger and more general patient population could help us to clarify whether diet, time of the day at sampling, tumours of the upper GI tract and so on can affect faecal MMP-9 levels and could also serve to verify the performance of our method. A comparison with FIT on the same samples could be useful. Moreover, a larger study on a general cohort could also provide more information if this method is valuable for CRC screening.

Nonetheless, our study was suitable to identify a new marker, faecal MMP-9, capable of discrimination of CRC from polyp-free controls. Faecal MMP-9 is characterised by many advantages of FIT and also of the molecular markers, without high costs. Further studies on a larger and more general population are needed to confirm whether this promising method is suitable for CRC screening as a complementary assay.