Ana Lilia Salas1, María Rosa Alberto1, Iris Catiana Zampini1, Ana Soledad Cuello1, Luis Maldonado2, José Luis Ríos3, Guillermo Schmeda-Hirschmann4, María Inés Isla5. 1. Laboratorio de Investigación de Productos Naturales (LIPRON), INQUINOA.CONICET. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Universidad Nacional de Tucumán, 4000 San Miguel de Tucumán, Argentina. 2. INTA, Famaillá, Tucumán, Argentina. 3. Departamento de Farmacología, Facultad de Farmacia, Universidad de Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, España. 4. Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Universidad de Talca, Casilla 747, 3460000 Talca, Chile. 5. Laboratorio de Investigación de Productos Naturales (LIPRON), INQUINOA.CONICET. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Universidad Nacional de Tucumán, 4000 San Miguel de Tucumán, Argentina. Electronic address: misla@tucbbs.com.ar.
Abstract
BACKGROUND: Propolis is a bioactive natural product collected by honeybees (Apis mellifera) from plant sources. PURPOSE: This study was undertaken to determine the effect of propolis extracts from arid region of Argentina, on the activity/expression of pro-inflammatory enzymes, and as potential free radical scavenger, antifungal and anthelmintic agent as well as to get a first insight into the polyphenolic profile of the active fractions. STUDY DESIGN/ METHODS: Two propolis samples were collected in different time from hives located in Tucumán, Argentina. They are representative of the collection time of the raw material for phytotherapeutical purposes. Ethanolic extracts from both propolis were obtained. The PEEs were analyzed for total polyphenol (TP), non-flavonoid phenols (NFP) and flavonoid (FP) content followed by HPLC-DAD analysis and identification of components by HPLC-MS/MS(n). The potentiality as anti-inflammatory (LOX, COX, iNOS enzymes), antioxidant, antifungal and nematicidal was determined. RESULTS: PEEs contain high levels of TP, NFP and FP, including cinnamic acid, caffeic acid prenyl ester, caffeoyl dihydrocaffeate and caffeic acid 3,4-dihydroxyphenethyl ester, liquiritigenin, 2',4'-dihydroxychalcone and 2',4'-dihydroxy-3'-methoxychalcone. The PEEs in vitro reduced the activity of LOX and COX-2. Pretreatment of RAW 264.7 cells with PEEs before the induction of inflammatory state, inhibited NO overproduction and the iNOS protein expression was significantly decreased. The PEEs exhibited antioxidant, antifungal (Candida sp.) and nematicidal effect (C. elegans). CONCLUSION: These findings show the potential use of characterized PEEs from arid regions of Argentina as phytomedicine.
BACKGROUND: Propolis is a bioactive natural product collected by honeybees (Apis mellifera) from plant sources. PURPOSE: This study was undertaken to determine the effect of propolis extracts from arid region of Argentina, on the activity/expression of pro-inflammatory enzymes, and as potential free radical scavenger, antifungal and anthelmintic agent as well as to get a first insight into the polyphenolic profile of the active fractions. STUDY DESIGN/ METHODS: Two propolis samples were collected in different time from hives located in Tucumán, Argentina. They are representative of the collection time of the raw material for phytotherapeutical purposes. Ethanolic extracts from both propolis were obtained. The PEEs were analyzed for total polyphenol (TP), non-flavonoidphenols (NFP) and flavonoid (FP) content followed by HPLC-DAD analysis and identification of components by HPLC-MS/MS(n). The potentiality as anti-inflammatory (LOX, COX, iNOS enzymes), antioxidant, antifungal and nematicidal was determined. RESULTS:PEEs contain high levels of TP, NFP and FP, including cinnamic acid, caffeic acid prenyl ester, caffeoyl dihydrocaffeate and caffeic acid 3,4-dihydroxyphenethyl ester, liquiritigenin, 2',4'-dihydroxychalcone and 2',4'-dihydroxy-3'-methoxychalcone. The PEEs in vitro reduced the activity of LOX and COX-2. Pretreatment of RAW 264.7 cells with PEEs before the induction of inflammatory state, inhibited NO overproduction and the iNOS protein expression was significantly decreased. The PEEs exhibited antioxidant, antifungal (Candida sp.) and nematicidal effect (C. elegans). CONCLUSION: These findings show the potential use of characterized PEEs from arid regions of Argentina as phytomedicine.
Authors: Islam Ali Abdel Raheem; Amro Abdul Razek; Abeer Abdelaziz Elgendy; Noha Mohamed Saleh; Mona Ibrahem Shaaban; Faten K Abd El-Hady Journal: Int J Nanomedicine Date: 2019-10-21