Literature DB >> 2690010

Sequence motifs characteristic of DNA[cytosine-N4]methyltransferases: similarity to adenine and cytosine-C5 DNA-methylases.

S Klimasauskas1, A Timinskas, S Menkevicius, D Butkienè, V Butkus, A Janulaitis.   

Abstract

The sequences coding for DNA[cytosine-N4]methyltransferases MvaI (from Micrococcus varians RFL19) and Cfr9I (from Citrobacter freundii RFL9) have been determined. The predicted methylases are proteins of 454 and 300 amino acids, respectively. Primary structure comparison of M.Cfr9I and another m4C-forming methylase, M.Pvu II, revealed extended regions of homology. The sequence comparison of the three DNA[cytosine-N4]-methylases using originally developed software revealed two conserved patterns, DPF-GSGT and TSPPY, which were found similar also to those of adenine and DNA[cytosine-C5]-methylases. These data provided a basis for global alignment and classification of DNA-methylase sequences. Structural considerations led us to suggest that the first region could be the binding site of AdoMet, while the second is thought to be directly involved in the modification of the exocyclic amino group.

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Year:  1989        PMID： 2690010      PMCID： PMC335216          DOI： 10.1093/nar/17.23.9823

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  44 in total

1.  Sequence, internal homology and high-level expression of the gene for a DNA-(cytosine N4)-methyltransferase, M.Pvu II.

Authors:  T Tao; J Walter; K J Brennan; M M Cotterman; R M Blumenthal
Journal:  Nucleic Acids Res       Date:  1989-06-12       Impact factor: 16.971

2.  DNA methyltransferase genes of Bacillus subtilis phages: comparison of their nucleotide sequences.

Authors:  A Tran-Betcke; B Behrens; M Noyer-Weidner; T A Trautner
Journal:  Gene       Date:  1986       Impact factor: 3.688

3.  Rapid and sensitive protein similarity searches.

Authors:  D J Lipman; W R Pearson
Journal:  Science       Date:  1985-03-22       Impact factor: 47.728

4.  Nucleotide sequence of the PaeR7 restriction/modification system and partial characterization of its protein products.

Authors:  G Theriault; P H Roy; K A Howard; J S Benner; J E Brooks; A F Waters; T R Gingeras
Journal:  Nucleic Acids Res       Date:  1985-12-09       Impact factor: 16.971

5.  N4-methylcytosine as a minor base in bacterial DNA.

Authors:  M Ehrlich; G G Wilson; K C Kuo; C W Gehrke
Journal:  J Bacteriol       Date:  1987-03       Impact factor: 3.490

6.  Cloning and characterization of the genes encoding the MspI restriction modification system.

Authors:  P M Lin; C H Lee; R J Roberts
Journal:  Nucleic Acids Res       Date:  1989-04-25       Impact factor: 16.971

7.  Nucleotide sequence of the Dpn II DNA methylase gene of Streptococcus pneumoniae and its relationship to the dam gene of Escherichia coli.

Authors:  B M Mannarelli; T S Balganesh; B Greenberg; S S Springhorn; S A Lacks
Journal:  Proc Natl Acad Sci U S A       Date:  1985-07       Impact factor: 11.205

8.  DNA methylation in thermophilic bacteria: N4-methylcytosine, 5-methylcytosine, and N6-methyladenine.

Authors:  M Ehrlich; M A Gama-Sosa; L H Carreira; L G Ljungdahl; K C Kuo; C W Gehrke
Journal:  Nucleic Acids Res       Date:  1985-02-25       Impact factor: 16.971

9.  Investigation of restriction-modification enzymes from M. varians RFL19 with a new type of specificity toward modification of substrate.

Authors:  V Butkus; S Klimasauskas; D Kersulyte; D Vaitkevicius; A Lebionka; A Janulaitis
Journal:  Nucleic Acids Res       Date:  1985-08-26       Impact factor: 16.971

10.  Regulation of a new bacteriophage T4 gene, 69, that spans an origin of DNA replication.

Authors:  P M Macdonald; G Mosig
Journal:  EMBO J       Date:  1984-12-01       Impact factor: 11.598
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  72 in total

1.  Specificities of eleven different DNA methyltransferases of Helicobacter pylori strain 26695.

Authors:  J Vitkute; K Stankevicius; G Tamulaitiene; Z Maneliene; A Timinskas; D E Berg; A Janulaitis
Journal:  J Bacteriol       Date:  2001-01       Impact factor: 3.490

Review 2.  Nucleoside triphosphate-dependent restriction enzymes.

Authors:  D T Dryden; N E Murray; D N Rao
Journal:  Nucleic Acids Res       Date:  2001-09-15       Impact factor: 16.971

3.  Substrate DNA and cofactor regulate the activities of a multi-functional restriction-modification enzyme, BcgI.

Authors:  H Kong; C L Smith
Journal:  Nucleic Acids Res       Date:  1997-09-15       Impact factor: 16.971

4.  REBASE: restriction enzymes and methyltransferases.

Authors:  Richard J Roberts; Tamas Vincze; Janos Posfai; Dana Macelis
Journal:  Nucleic Acids Res       Date:  2003-01-01       Impact factor: 16.971

5.  A new Thermus sp. class-IIS enzyme sub-family: isolation of a 'twin' endonuclease TspDTI with a novel specificity 5'-ATGAA(N(11/9))-3', related to TspGWI, TaqII and Tth111II.

Authors:  Piotr M Skowron; Jarosław Majewski; Agnieszka Zylicz-Stachula; Sylwia M Rutkowska; Izabela Jaworowska; Renata I Harasimowicz-Słowińska
Journal:  Nucleic Acids Res       Date:  2003-07-15       Impact factor: 16.971

6.  Purification and properties of the Eco57I restriction endonuclease and methylase--prototypes of a new class (type IV).

Authors:  A Janulaitis; M Petrusyte; Z Maneliene; S Klimasauskas; V Butkus
Journal:  Nucleic Acids Res       Date:  1992-11-25       Impact factor: 16.971

7.  Cloning and sequence analysis of the genes coding for Eco57I type IV restriction-modification enzymes.

Authors:  A Janulaitis; R Vaisvila; A Timinskas; S Klimasauskas; V Butkus
Journal:  Nucleic Acids Res       Date:  1992-11-25       Impact factor: 16.971

8.  How M.MspI and M.HpaII decide which base to methylate.

Authors:  S Mi; R J Roberts
Journal:  Nucleic Acids Res       Date:  1992-09-25       Impact factor: 16.971

9.  Alw26I, Eco31I and Esp3I--type IIs methyltransferases modifying cytosine and adenine in complementary strands of the target DNA.

Authors:  J Bitinaite; Z Maneliene; S Menkevicius; S Klimasauskas; V Butkus; A Janulaitis
Journal:  Nucleic Acids Res       Date:  1992-10-11       Impact factor: 16.971

10.  eps15, a novel tyrosine kinase substrate, exhibits transforming activity.

Authors:  F Fazioli; L Minichiello; B Matoskova; W T Wong; P P Di Fiore
Journal:  Mol Cell Biol       Date:  1993-09       Impact factor: 4.272
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