| Literature DB >> 26896350 |
Beibei Wang1, Jie Dong1, Jiaojiao Ji1, Jiang Yuan1, Jiali Wang1, Jiarui Wu1, Peng Tan1, Yonggang Liu2.
Abstract
A rapid, convenient and effective identification method of alkaloids was established and an attempt on isolating and analyzing the alkaloids in Aconitum pendulum Busch was conducted successfully. In this article, four high-content components including deoxyaconitine, benzoylaconine, aconine and neoline were isolated by using column chromatography. HPLC-MS(n)was employed to deduce the regulations of fragmentation of diterpenoid alkaloids which displayed a characteristic behavior of loss of CO(28u), CH3COOH(60u), CH3OH(32u), H2O(18u) and C6H5COOH(122u). Then, according to fragmentation regulation of mass spectrometry, 42 alkaloids were found inA. pendulum Among them, 38 compounds were identified and 29 alkaloids were reported for the first time for this herb. Therefore, this means that HPLC-MS(n)combined with column chromatography could work as an effective and reliable tool for rapid identification of the chemical components of herbal medicine.Entities:
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Year: 2016 PMID： 26896350 PMCID： PMC4890449 DOI： 10.1093/chromsci/bmw002
Source DB: PubMed Journal: J Chromatogr Sci ISSN： 0021-9665 Impact factor: 1.618