Jong Hee Hyun1, Seok-Ki Kim2, Kwang Gi Kim3, Hong Rae Kim3, Hyun Min Lee3, Sunup Park2, Sung Chun Kim2, Yongdoo Choi2, Dae Kyung Sohn4,5. 1. Center for Colorectal Cancer, Research Institute and Hospital, National Cancer Center, 323 Ilsan-ro, Ilsandong-gu, Goyang-Si, Gyeonggi-do, 410-769, Republic of Korea. 2. Molecular Imaging and Therapy Branch, Research Institute, National Cancer Center, Goyang, Republic of Korea. 3. Biomedical Engineering Branch, Research Institute, National Cancer Center, Goyang, Republic of Korea. 4. Center for Colorectal Cancer, Research Institute and Hospital, National Cancer Center, 323 Ilsan-ro, Ilsandong-gu, Goyang-Si, Gyeonggi-do, 410-769, Republic of Korea. gsgabal@ncc.re.kr. 5. Biomedical Engineering Branch, Research Institute, National Cancer Center, Goyang, Republic of Korea. gsgabal@ncc.re.kr.
Abstract
BACKGROUND: Accurate tumor localization is essential for minimally invasive surgery. This study describes the development of a novel endoscopic fluorescent band ligation method for the rapid and accurate identification of tumor sites during surgery. METHODS AND MATERIALS: The method utilized a fluorescent rubber band, made of indocyanine green (ICG) and a liquid rubber solution mixture, as well as a near-infrared fluorescence laparoscopic system with a dual light source using a high-powered light-emitting diode (LED) and a 785-nm laser diode. The fluorescent rubber bands were endoscopically placed on the mucosae of porcine stomachs and colons. During subsequent conventional laparoscopic stomach and colon surgery, the fluorescent bands were assayed using the near-infrared fluorescence laparoscopy system. RESULTS: The locations of the fluorescent clips were clearly identified on the fluorescence images in real time. The system was able to distinguish the two or three bands marked on the mucosal surfaces of the stomach and colon. Resection margins around the fluorescent bands were sufficient in the resected specimens obtained during stomach and colon surgery. CONCLUSION: These novel endoscopic fluorescent bands could be rapidly and accurately localized during stomach and colon surgery. Use of these bands may make possible the excision of exact target sites during minimally invasive gastrointestinal surgery.
BACKGROUND: Accurate tumor localization is essential for minimally invasive surgery. This study describes the development of a novel endoscopic fluorescent band ligation method for the rapid and accurate identification of tumor sites during surgery. METHODS AND MATERIALS: The method utilized a fluorescent rubber band, made of indocyanine green (ICG) and a liquid rubber solution mixture, as well as a near-infrared fluorescence laparoscopic system with a dual light source using a high-powered light-emitting diode (LED) and a 785-nm laser diode. The fluorescent rubber bands were endoscopically placed on the mucosae of porcine stomachs and colons. During subsequent conventional laparoscopic stomach and colon surgery, the fluorescent bands were assayed using the near-infrared fluorescence laparoscopy system. RESULTS: The locations of the fluorescent clips were clearly identified on the fluorescence images in real time. The system was able to distinguish the two or three bands marked on the mucosal surfaces of the stomach and colon. Resection margins around the fluorescent bands were sufficient in the resected specimens obtained during stomach and colon surgery. CONCLUSION: These novel endoscopic fluorescent bands could be rapidly and accurately localized during stomach and colon surgery. Use of these bands may make possible the excision of exact target sites during minimally invasive gastrointestinal surgery.
Authors: N Price; M R Gottfried; E Clary; D C Lawson; J Baillie; K Mergener; C Westcott; S Eubanks; T N Pappas Journal: Gastrointest Endosc Date: 2000-04 Impact factor: 9.427
Authors: J W Park; D K Sohn; C W Hong; K S Han; D H Choi; H J Chang; S-B Lim; H S Choi; S-Y Jeong Journal: Surg Endosc Date: 2007-08-17 Impact factor: 4.584