Jukka Niskanen1, Issan Zhang2, Yanming Xue3, Dmitri Golberg3, Dusica Maysinger2, Françoise M Winnik1,3. 1. Université de Montréal, Faculté de Pharmacie et Département de Chimie, CP 6128 Succursale Centre Ville, Montréal, QC, H3C 3J7, Canada. 2. Department of Pharmacology & Therapeutics, Faculty of Medicine, McGill University, 3655 Promenade Sir-William-Osler, H3G 1Y6, Montréal, Québec, Canada. 3. International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
Abstract
AIM: To evaluate the response of cells to boron nitride nanotubes (BNNTs) carrying fluorescent probes or drugs in their inner channel by assessment of the cellular localization of the fluorescent cargo, evaluation of the in vitro release and biological activity of a drug (curcumin) loaded in BNNTs. METHODS: Cells treated with curcumin-loaded BNNTs and stimulated with lipopolysaccharide were assessed for nitric oxide release and stimulation of IL-6 and TNF-α. The cellular trafficking of two cell-permeant dyes and a non-cell-permeant dye loaded within BNNTs was imaged. RESULTS: BNNTs loaded with up to 13 wt% fluorophores were internalized by cells and controlled release of curcumin triggered cellular pathways associated with the known anti-inflammatory effects of the drug. CONCLUSION: The overall findings indicate that BNNTs can function as nanocarriers of biologically relevant probes/drugs allowing one to examine/control their local intracellular localization and biochemical effects, leading the way to applications as intracellular nanosensors.
AIM: To evaluate the response of cells to boron nitride nanotubes (BNNTs) carrying fluorescent probes or drugs in their inner channel by assessment of the cellular localization of the fluorescent cargo, evaluation of the in vitro release and biological activity of a drug (curcumin) loaded in BNNTs. METHODS: Cells treated with curcumin-loaded BNNTs and stimulated with lipopolysaccharide were assessed for nitric oxide release and stimulation of IL-6 and TNF-α. The cellular trafficking of two cell-permeant dyes and a non-cell-permeant dye loaded within BNNTs was imaged. RESULTS:BNNTs loaded with up to 13 wt% fluorophores were internalized by cells and controlled release of curcumin triggered cellular pathways associated with the known anti-inflammatory effects of the drug. CONCLUSION: The overall findings indicate that BNNTs can function as nanocarriers of biologically relevant probes/drugs allowing one to examine/control their local intracellular localization and biochemical effects, leading the way to applications as intracellular nanosensors.
Authors: Ashleigh D Smith McWilliams; Carlos A de Los Reyes; Lucy Liberman; Selin Ergülen; Yeshayahu Talmon; Matteo Pasquali; Angel A Martí Journal: Nanoscale Adv Date: 2018-12-17