Wenju Li1, Haifeng Wei2, Chunmei Xia3, Xiaomeng Zhu1, Guozhu Hou4, Feng Xu5, Xinghua Song1, Yulin Zhan6. 1. Department of Orthopedics, Xinjiang 1st Teaching Hospital, Xinjiang Medical College Urumuqi 830011, Xinjiang, China. 2. Department of Orthopedics, Shanghai Sixth people's Hospital, Jiaotong University Shanghai 200032, China. 3. Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University Shanghai 200032, China. 4. Department of Emergency, General Hospital of Pingmei Shenma Medical Group Pingdingshan 467000, Henan, China. 5. Department of Orthopedics, Fifth Hospital of Wuhan, Second Affiliated Hospital of Jianghan University Wuhan 430050, Hubei, China. 6. Department of Orthopedics, Xinjiang 1st Teaching Hospital, Xinjiang Medical CollegeUrumuqi 830011, Xinjiang, China; Department of Orthopedics, Shanghai Sixth people's Hospital, Jiaotong UniversityShanghai 200032, China.
Abstract
PURPOSE: Transferring the bone morphogenetic protein 2 (BMP-2) genes into the tissues or cells can improve the bone healing of the fracture has been widely accepted. We evaluated the efficiency of using gene gun to transfer the BMP-2 gene thereby affected the healing of a fractured bone. METHODS: The vector coding for BMP-2 was constructed by a non-replicating encephalo-myocarditis virus (ECMV)-based vector. The segmental bone defect (1.5 cm) model was created by a wire-saw at the middle part of the radius bone of the New Zealand white rabbits. Then either BMP-2 gene or control vector without BMP-2 gene was injected into the tissues around the fracture site. Healing of the defects was monitored radiographically for 9 weeks, bone consolidation was determined by the Lane-Sandhu score pre- and post-operatively, which can evaluated bone formation, bone connect and bone plasticity. RESULTS: The radiographic score and bone consolidation rates were significantly higher in animals injected with BMP-2 gene group as compared with control vector-injected animals (P<0.05). The control group still showed no radiological signs of stable healing. Western-blot and RT-PCR showed BMP-2 expression was significant increase in the tissues around the site of osseous lesions in comparison with the control vector-injected animals (P<0.05). CONCLUSIONS: Our results suggested that BMP-2 gene transferred by gene gun could increase the expression of BMP-2 protein and improved the bone callus formation therefore shortened the time of bone defect healing.
PURPOSE: Transferring the bone morphogenetic protein 2 (BMP-2) genes into the tissues or cells can improve the bone healing of the fracture has been widely accepted. We evaluated the efficiency of using gene gun to transfer the BMP-2 gene thereby affected the healing of a fractured bone. METHODS: The vector coding for BMP-2 was constructed by a non-replicating encephalo-myocarditis virus (ECMV)-based vector. The segmental bone defect (1.5 cm) model was created by a wire-saw at the middle part of the radius bone of the New Zealand white rabbits. Then either BMP-2 gene or control vector without BMP-2 gene was injected into the tissues around the fracture site. Healing of the defects was monitored radiographically for 9 weeks, bone consolidation was determined by the Lane-Sandhu score pre- and post-operatively, which can evaluated bone formation, bone connect and bone plasticity. RESULTS: The radiographic score and bone consolidation rates were significantly higher in animals injected with BMP-2 gene group as compared with control vector-injected animals (P<0.05). The control group still showed no radiological signs of stable healing. Western-blot and RT-PCR showed BMP-2 expression was significant increase in the tissues around the site of osseous lesions in comparison with the control vector-injected animals (P<0.05). CONCLUSIONS: Our results suggested that BMP-2 gene transferred by gene gun could increase the expression of BMP-2 protein and improved the bone callus formation therefore shortened the time of bone defect healing.
Entities:
Keywords:
Bone morphogenetic protein 2; bone fracture; gene gun; healing
Authors: A W Baltzer; C Lattermann; J D Whalen; P Wooley; K Weiss; M Grimm; S C Ghivizzani; P D Robbins; C H Evans Journal: Gene Ther Date: 2000-05 Impact factor: 5.250
Authors: Suzanne N Lissenberg-Thunnissen; David J J de Gorter; Cornelis F M Sier; Inger B Schipper Journal: Int Orthop Date: 2011-06-23 Impact factor: 3.075