Yan-Ming Tian1, Yan Liu2, Sheng Wang1, Yi Dong3, Tong Su3, Hui-Jie Ma4, Yi Zhang5. 1. Department of Physiology, Hebei Medical University, Shijiazhuang 050017, PR China; Hebei Collaborative Innovation Center for Cardio-cerebrovascular Disease, Shijiazhuang 050000, PR China. 2. Department of Endocrinology, The Third Hospital of Hebei Medical University, Shijiazhuang 050051, PR China. 3. Department of Physiology, Hebei Medical University, Shijiazhuang 050017, PR China. 4. Department of Physiology, Hebei Medical University, Shijiazhuang 050017, PR China; Hebei Collaborative Innovation Center for Cardio-cerebrovascular Disease, Shijiazhuang 050000, PR China. Electronic address: lily564300@163.com. 5. Department of Physiology, Hebei Medical University, Shijiazhuang 050017, PR China; Hebei Collaborative Innovation Center for Cardio-cerebrovascular Disease, Shijiazhuang 050000, PR China. Electronic address: zhyhenryphy@163.com.
Abstract
AIM: Cumulating evidence demonstrated that chronic intermittent hypobaric hypoxia (CIHH) had beneficial effects on the body. The present study was to investigate the anti-diabetes effect of CIHH in type-2 diabetic rats for the first time. MAIN METHODS: Sprague-Dawley rats were randomly divided into 4 groups: control group (CON), diabetes mellitus group (DM, induced by high-fat diet combined with low-dose streptozotocin), CIHH treatment group (CIHH, simulated 5000-m altitude, 6h per day for 28 days), and diabetes mellitus plus CIHH treatment group (DM+CIHH). Histopathology of liver, systolic arterial blood pressure (SAP), blood biochemicals, glucose and insulin tolerance were determined. The expression of proteins associated with insulin signaling pathway as well as hypoxia induced factors were assayed. KEY FINDINGS: Diabetic rats showed impaired glucose tolerance, dyslipidemia, hepatic steatosis and hepatic insulin resistance in addition to increased SAP. However, SAP, serum triglyceride and cholesterol were decreased, and hepatic steatosis and insulin resistance were improved in DM+CIHH rats. Furthermore, the protein expression of glucokinase (GCK), insulin receptor substrates (IRS-1 and IRS-2), and HIF1α were increased, while the expression of phosphoenolpyruvate carboxykinase (PEPCK), was markedly reduced in DM+CIHH rats. SIGNIFICANCE: We conclude that CIHH treatment has anti-diabetes effects through ameliorating insulin resistance via hepatic HIF-insulin signaling pathway in type-2 diabetic rats.
AIM: Cumulating evidence demonstrated that chronic intermittent hypobaric hypoxia (CIHH) had beneficial effects on the body. The present study was to investigate the anti-diabetes effect of CIHH in type-2 diabeticrats for the first time. MAIN METHODS:Sprague-Dawley rats were randomly divided into 4 groups: control group (CON), diabetes mellitus group (DM, induced by high-fat diet combined with low-dose streptozotocin), CIHH treatment group (CIHH, simulated 5000-m altitude, 6h per day for 28 days), and diabetes mellitus plus CIHH treatment group (DM+CIHH). Histopathology of liver, systolic arterial blood pressure (SAP), blood biochemicals, glucose and insulin tolerance were determined. The expression of proteins associated with insulin signaling pathway as well as hypoxia induced factors were assayed. KEY FINDINGS:Diabeticrats showed impaired glucose tolerance, dyslipidemia, hepatic steatosis and hepatic insulin resistance in addition to increased SAP. However, SAP, serum triglyceride and cholesterol were decreased, and hepatic steatosis and insulin resistance were improved in DM+CIHHrats. Furthermore, the protein expression of glucokinase (GCK), insulin receptor substrates (IRS-1 and IRS-2), and HIF1α were increased, while the expression of phosphoenolpyruvate carboxykinase (PEPCK), was markedly reduced in DM+CIHHrats. SIGNIFICANCE: We conclude that CIHH treatment has anti-diabetes effects through ameliorating insulin resistance via hepatic HIF-insulin signaling pathway in type-2 diabeticrats.
Authors: Tetiana V Serebrovska; Alla G Portnychenko; Tetiana I Drevytska; Vladimir I Portnichenko; Lei Xi; Egor Egorov; Anna V Gavalko; Svitlana Naskalova; Valentina Chizhova; Valeriy B Shatylo Journal: Exp Biol Med (Maywood) Date: 2017-07-31
Authors: Robert T Mallet; Eugenia B Manukhina; Steven Shea Ruelas; James L Caffrey; H Fred Downey Journal: Am J Physiol Heart Circ Physiol Date: 2018-04-13 Impact factor: 4.733
Authors: Tetiana V Serebrovska; Alla G Portnychenko; Vladimir I Portnichenko; Lei Xi; Egor Egorov; Ivanna Antoniuk-Shcheglova; Svitlana Naskalova; Valeriy B Shatylo Journal: Eur J Appl Physiol Date: 2019-01-30 Impact factor: 3.078