Literature DB >> 26880717

Giardia and Cryptosporidium spp. dissemination during wastewater treatment and comparative detection via immunofluorescence assay (IFA), nested polymerase chain reaction (nested PCR) and loop mediated isothermal amplification (LAMP).

Carmen Gallas-Lindemann1, Isaia Sotiriadou2, Judit Plutzer3, Michael J Noack4, Mohammad Reza Mahmoudi5, Panagiotis Karanis6.   

Abstract

Environmental water samples from the Lower Rhine area in Germany were investigated via immunofluorescence assays (IFAs), nested polymerase chain reaction (nested PCR) and loop-mediated isothermal amplification (LAMP) to detect the presence of Giardia spp. (n=185) and Cryptosporidium spp. (n=227). The samples were concentrated through filtration or flocculation, and oocysts were purified via centrifugation through a sucrose density gradient. For all samples, IFA was performed first, followed by DNA extraction for the nested PCR and LAMP assays. Giardia cysts were detected in 105 samples (56.8%) by IFA, 62 samples (33.5%) by nested PCR and 79 samples (42.7%) by LAMP. Cryptosporidium spp. were detected in 69 samples (30.4%) by IFA, 95 samples (41.9%) by nested PCR and 99 samples (43.6%) by LAMP. According to these results, the three detection methods are complementary for monitoring Giardia and Cryptosporidium in environmental waters.
Copyright © 2016 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Cryptosporidium; Detection; Dissemination; Giardia; LAMP; Wastewater

Mesh：

Substances：

Year:  2016        PMID： 26880717     DOI： 10.1016/j.actatropica.2016.02.005

Source DB:  PubMed          Journal:  Acta Trop        ISSN： 0001-706X            Impact factor:   3.112


  7 in total

1.  Giardia spp. and Cryptosporidium spp. removal efficiency of a combined fixed-film system treating domestic wastewater receiving hospital effluent.

Authors:  Sandra Yamashiro; Mário Luiz Rodrigues Foco; Carolina Ortiz Pineda; Juliana José; Edson Aparecido Abdul Nour; Isabel Cristina Vidal Siqueira-Castro; Regina Maura Bueno Franco
Journal:  Environ Sci Pollut Res Int       Date:  2019-06-06       Impact factor: 4.223

Review 2.  Prevalence of Cryptosporidium and Giardia in vegetables in Iran: a nineteen-years meta-analysis review.

Authors:  Ehsan Javanmard; Elnaz Sadat Mirsamadi; Meysam Olfatifar; Erfan Ghasemi; Fatemeh Saki; Hamed Mirjalali; Mohammad Reza Zali; Panagiotis Karanis
Journal:  J Environ Health Sci Eng       Date:  2020-06-18

3.  Molecular diagnosis of protozoan parasites by Recombinase Polymerase Amplification.

Authors:  A Castellanos-Gonzalez; A C White; P Melby; B Travi
Journal:  Acta Trop       Date:  2018-02-13       Impact factor: 3.112

4.  Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers: Detection of Cryptosporidium Species in Kenyan Children Presenting with Diarrhea.

Authors:  Timothy S Mamba; Cecilia K Mbae; Johnson Kinyua; Erastus Mulinge; Gitonga Nkanata Mburugu; Zablon K Njiru
Journal:  J Trop Med       Date:  2018-02-26

Review 5.  Detection of Cryptosporidium spp. and Giardia spp. in Environmental Water Samples: A Journey into the Past and New Perspectives.

Authors:  Marie-Stéphanie Fradette; Alexander I Culley; Steve J Charette
Journal:  Microorganisms       Date:  2022-06-07

6.  Comparative Performance of Eight PCR Methods to Detect Cryptosporidium Species.

Authors:  Damien Costa; Louise Soulieux; Romy Razakandrainibe; Louise Basmaciyan; Gilles Gargala; Stéphane Valot; Frédéric Dalle; Loic Favennec
Journal:  Pathogens       Date:  2021-05-23

Review 7.  Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications.

Authors:  Syahidah Nurani Zulkifli; Herlina Abdul Rahim; Woei-Jye Lau
Journal:  Sens Actuators B Chem       Date:  2017-09-18       Impact factor: 7.460
  7 in total

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