Literature DB >> 26880106

Modified RNAs in CRISPR/Cas9: An Old Trick Works Again.

Alfonso Latorre1, Ana Latorre1, Álvaro Somoza2.   

Abstract

Old tricks, new dog: CRISPR/Cas9 is a powerful tool for gene editing that requires an endonuclease (Cas9) and RNA strands. It has been shown that chemical modification of the RNA structures, an approach that has been used to improve the efficiency of RNA interference, can also be applied to enhance the activity of CRISPR/Cas9 and reduce its off-target effects.
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  CRISPR/Cas; RNA; gene editing; nucleotide analogues; nucleotides

Mesh：

Substances：

Year:  2016        PMID： 26880106     DOI： 10.1002/anie.201512002

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  6 in total

1.  Concurrent Hydrogenation of Three Functional Groups Enables Synthesis of C3'-Homologated Nucleoside Amino Acids.

Authors:  Venubabu Kotikam; Eriks Rozners
Journal:  Org Lett       Date:  2017-07-21       Impact factor: 6.005

2.  Synthetic Oligonucleotides Inhibit CRISPR-Cpf1-Mediated Genome Editing.

Authors:  Bin Li; Chunxi Zeng; Wenqing Li; Xinfu Zhang; Xiao Luo; Weiyu Zhao; Chengxiang Zhang; Yizhou Dong
Journal:  Cell Rep       Date:  2018-12-18       Impact factor: 9.423

Review 3.  Modified Nucleic Acids: Expanding the Capabilities of Functional Oligonucleotides.

Authors:  Steven Ochoa; Valeria T Milam
Journal:  Molecules       Date:  2020-10-13       Impact factor: 4.411

Review 4.  Delivering the CRISPR/Cas9 system for engineering gene therapies: Recent cargo and delivery approaches for clinical translation.

Authors:  Ruth A Foley; Ruby A Sims; Emily C Duggan; Jessica K Olmedo; Rachel Ma; Steven J Jonas
Journal:  Front Bioeng Biotechnol       Date:  2022-09-26

5.  Amide linkages mimic phosphates in RNA interactions with proteins and are well tolerated in the guide strand of short interfering RNAs.

Authors:  Daniel Mutisya; Travis Hardcastle; Samwel K Cheruiyot; Pradeep S Pallan; Scott D Kennedy; Martin Egli; Melissa L Kelley; Anja van Brabant Smith; Eriks Rozners
Journal:  Nucleic Acids Res       Date:  2017-08-21       Impact factor: 16.971

6.  From Traditional Breeding to Genome Editing for Boosting Productivity of the Ancient Grain Tef [Eragrostis tef (Zucc.) Trotter].

Authors:  Muhammad Numan; Abdul Latif Khan; Sajjad Asaf; Mohammad Salehin; Getu Beyene; Zerihun Tadele; Ayalew Ligaba-Osena
Journal:  Plants (Basel)       Date:  2021-03-25
  6 in total

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