PURPOSE: In patients with active rheumatoid arthritis (RA) decrease of galactosylation is correlated with disease activity. The aim of our study was to evaluate an effect of methotrexate therapy on glycosylation disturbances of IgG in RA patients. MATERIALS/ METHODS: IgG glycosylation in 40 patients with active RA treated with methotrexate for 12 months prior to and after treatment were compared. The control group consisted of 20 healthy volunteers. IgG glycosylation was assessed using biotinylated lectins and immunosorbent ELISA assay. For galactose specificity Datura stramonium lectin (DSA), for sialic acid Sambucus nigra (SNA) and Maackia amurensis (MAA) and for fucose residue Areulia auranta (AAA) lectins were used. RESULTS: In RA-cases N-glycan galactosylation and sialylation of IgG before treatment were significantly lower than in healthy subjects (for DSA, MAA lectins p<0.001 and SNA p<0.05). Significant increase of IgG galactosylation and sialylation in RA patients after therapy (for DSA, MAA and SNA lectin p<0.05) was detected. Moreover the glycosylation disturbances of N-glycan IgG were strongly associated with changes of disease activity based on disease activity score. For fucose residues significantly higher absorbency of AAA lectin in RA patients before treatment was observed compared to control subjects (p<0.05) and slightly, not significantly decreased after MTX therapy. CONCLUSIONS: Defect of galactosylation of IgG in RA patients is a useful marker of disease activity that may be used for the assessment of therapy effectiveness. The role of IgG fucosylation and sialylation in RA pathogenesis has still to be determined.
PURPOSE: In patients with active rheumatoid arthritis (RA) decrease of galactosylation is correlated with disease activity. The aim of our study was to evaluate an effect of methotrexate therapy on glycosylation disturbances of IgG in RApatients. MATERIALS/ METHODS: IgG glycosylation in 40 patients with active RA treated with methotrexate for 12 months prior to and after treatment were compared. The control group consisted of 20 healthy volunteers. IgG glycosylation was assessed using biotinylated lectins and immunosorbent ELISA assay. For galactose specificity Datura stramonium lectin (DSA), for sialic acidSambucus nigra (SNA) and Maackia amurensis (MAA) and for fucose residue Areulia auranta (AAA) lectins were used. RESULTS: In RA-cases N-glycan galactosylation and sialylation of IgG before treatment were significantly lower than in healthy subjects (for DSA, MAA lectins p<0.001 and SNA p<0.05). Significant increase of IgG galactosylation and sialylation in RApatients after therapy (for DSA, MAA and SNA lectin p<0.05) was detected. Moreover the glycosylation disturbances of N-glycan IgG were strongly associated with changes of disease activity based on disease activity score. For fucose residues significantly higher absorbency of AAA lectin in RApatients before treatment was observed compared to control subjects (p<0.05) and slightly, not significantly decreased after MTX therapy. CONCLUSIONS: Defect of galactosylation of IgG in RApatients is a useful marker of disease activity that may be used for the assessment of therapy effectiveness. The role of IgG fucosylation and sialylation in RA pathogenesis has still to be determined.
Authors: Dieter Vanderschaeghe; Leander Meuris; Tom Raes; Hendrik Grootaert; Annelies Van Hecke; Xavier Verhelst; Frederique Van de Velde; Bruno Lapauw; Hans Van Vlierberghe; Nico Callewaert Journal: Mol Cell Proteomics Date: 2018-09-06 Impact factor: 5.911
Authors: Xing Zhou; Francesca Motta; Carlo Selmi; William M Ridgway; M Eric Gershwin; Weici Zhang Journal: Autoimmun Rev Date: 2021-03-14 Impact factor: 9.754
Authors: Susanna L Lundström; Aase H Hensvold; Dorothea Rutishauser; Lars Klareskog; A Jimmy Ytterberg; Roman A Zubarev; Anca I Catrina Journal: Arthritis Res Ther Date: 2017-08-09 Impact factor: 5.156
Authors: Claudio Franceschi; Paolo Garagnani; Cristina Morsiani; Maria Conte; Aurelia Santoro; Andrea Grignolio; Daniela Monti; Miriam Capri; Stefano Salvioli Journal: Front Med (Lausanne) Date: 2018-03-12
Authors: Elena Pipi; Saba Nayar; David H Gardner; Serena Colafrancesco; Charlotte Smith; Francesca Barone Journal: Front Immunol Date: 2018-09-12 Impact factor: 7.561
Authors: Ema Prenc; Drazen Pulanic; Maja Pucic-Bakovic; Ivo Ugrina; Lana Desnica; Milan Milosevic; Filip Pirsl; Sandra Mitchell; Jeremy Rose; Radovan Vrhovac; Damir Nemet; Gordan Lauc; Steven Z Pavletic Journal: Front Immunol Date: 2021-07-14 Impact factor: 7.561