| Literature DB >> 26863196 |
Dan Dominissini1,2, Sigrid Nachtergaele1,2, Sharon Moshitch-Moshkovitz3, Eyal Peer3,4, Nitzan Kol3, Moshe Shay Ben-Haim3,4, Qing Dai1,2, Ayelet Di Segni3, Mali Salmon-Divon3, Wesley C Clark5, Guanqun Zheng5, Tao Pan5, Oz Solomon3,6, Eran Eyal3, Vera Hershkovitz3, Dali Han1,2, Louis C Doré1,2, Ninette Amariglio3,6, Gideon Rechavi3,4, Chuan He1,2,5.
Abstract
Gene expression can be regulated post-transcriptionally through dynamic and reversible RNA modifications. A recent noteworthy example is N(6)-methyladenosine (m(6)A), which affects messenger RNA (mRNA) localization, stability, translation and splicing. Here we report on a new mRNA modification, N(1)-methyladenosine (m(1)A), that occurs on thousands of different gene transcripts in eukaryotic cells, from yeast to mammals, at an estimated average transcript stoichiometry of 20% in humans. Employing newly developed sequencing approaches, we show that m(1)A is enriched around the start codon upstream of the first splice site: it preferentially decorates more structured regions around canonical and alternative translation initiation sites, is dynamic in response to physiological conditions, and correlates positively with protein production. These unique features are highly conserved in mouse and human cells, strongly indicating a functional role for m(1)A in promoting translation of methylated mRNA.Entities:
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Year: 2016 PMID： 26863196 PMCID： PMC4842015 DOI： 10.1038/nature16998
Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962