Literature DB >> 2685754

The bacteriophage T2 and T4 DNA-[N6-adenine] methyltransferase (Dam) sequence specificities are not identical.

S L Schlagman1, S Hattman.   

Abstract

Bacteriophages T2 and T4 encode DNA-[N6-adenine] methyltransferases (Dam) which differ from each other by only three amino acids. The canonical recognition sequence for these enzymes in both cytosine and 5-hydroxymethylcytosine-containing DNA is GATC; at a lower efficiency they also recognize some non-canonical sites in sequences derived from GAY (where Y is cytosine or thymine). We found that T4 Dam fails to methylate certain GATA and GATT sequences which are methylated by T2 Dam. This indicates that T2 Dam and T4 Dam do not have identical sequence specificities. We analyzed DNA sequence data files obtained from GenBank, containing about 30% of the T4 genome, to estimate the overall frequency of occurrence of GATC, as well as non-canonical sites derived from GAY. The observed N6methyladenine (m6A) content of T4 DNA, methylated exclusively at GATC (by Escherichia coli Dam), was found to be in good agreement with this estimate. Although GATC is fully methylated in virion DNA, only a small percentage of the non-canonical sequences are methylated.

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Year:  1989        PMID： 2685754      PMCID： PMC335117          DOI： 10.1093/nar/17.22.9101

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  30 in total

1.  Specificity of deoxyribonucleic acid transmethylase induced by bacteriophage T2. I. Nucleotide sequences isolated from tmicrococcus luteus DNA methylated in vitro.

Authors:  H van Ormondt; J Gorter; K J Havelaar; A de Waard
Journal:  Nucleic Acids Res       Date:  1975-08       Impact factor: 16.971

2.  THE ENZYMATIC METHYLATION OF RNA AND DNA. 8. EFFECTS OF BACTERIOPHAGE INFECTION ON THE ACTIVITY OF THE METHYLATING ENZYMES.

Authors:  M GOLD; R HAUSMANN; U MAITRA; J HURWITZ
Journal:  Proc Natl Acad Sci U S A       Date:  1964-08       Impact factor: 11.205

3.  Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation.

Authors:  S Lacks; B Greenberg
Journal:  J Mol Biol       Date:  1977-07       Impact factor: 5.469

4.  Electron microscope heteroduplex study of sequence relations of T2, T4, and T6 bacteriophage DNAs.

Authors:  J S Kim; N Davidson
Journal:  Virology       Date:  1974-01       Impact factor: 3.616

5.  Mutants of T2gt with altered DNA methylase activity: relation to restriction by prophage P1.

Authors:  H R Revel; S M Hattman
Journal:  Virology       Date:  1971-08       Impact factor: 3.616

6.  Mutants of bacteriophage T2 gt with altered DNA methylase activity.

Authors:  R Hehlmann; S Hattman
Journal:  J Mol Biol       Date:  1972-06-28       Impact factor: 5.469

7.  The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. IX. Deoxyribonucleic acid methylase in bacteriophage-infected Escherichia coli.

Authors:  R Hausmann; M Gold
Journal:  J Biol Chem       Date:  1966-05-10       Impact factor: 5.157

8.  DNA methylation of T-even bacteriophages and of their nonglucosylated mutants: its role in P1-directed restriction.

Authors:  S Hattman
Journal:  Virology       Date:  1970-10       Impact factor: 3.616

Review 9.  The effect of site-specific DNA methylation on restriction endonucleases and DNA modification methyltransferases--a review.

Authors:  M McClelland; M Nelson
Journal:  Gene       Date:  1988-12-25       Impact factor: 3.688

10.  The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. X. Bacteriophage T3-induced S-adenosylmethionine cleavage.

Authors:  M Gefter; R Hausmann; M Gold; J Hurwitz
Journal:  J Biol Chem       Date:  1966-05-10       Impact factor: 5.157
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  10 in total

1.  Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

Authors:  M McClelland; M Nelson
Journal:  Nucleic Acids Res       Date:  1992-05-11       Impact factor: 16.971

2.  Overexpression of the wild-type gene coding for Escherichia coli DNA adenine methylase (dam).

Authors:  V U Nwosu
Journal:  Biochem J       Date:  1992-05-01       Impact factor: 3.857

3.  Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.

Authors:  M Nelson; M McClelland
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

4.  Background Mutational Features of the Radiation-Resistant Bacterium Deinococcus radiodurans.

Authors:  Hongan Long; Sibel Kucukyildirim; Way Sung; Emily Williams; Heewook Lee; Matthew Ackerman; Thomas G Doak; Haixu Tang; Michael Lynch
Journal:  Mol Biol Evol       Date:  2015-05-14       Impact factor: 16.240

5.  Effect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases.

Authors:  M Nelson; E Raschke; M McClelland
Journal:  Nucleic Acids Res       Date:  1993-07-01       Impact factor: 16.971

6.  Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases.

Authors:  M McClelland; M Nelson; E Raschke
Journal:  Nucleic Acids Res       Date:  1994-09       Impact factor: 16.971

7.  Pre-steady state kinetics of bacteriophage T4 dam DNA-[N(6)-adenine] methyltransferase: interaction with native (GATC) or modified sites.

Authors:  E G Malygin; W M Lindstrom; S L Schlagman; S Hattman; N O Reich
Journal:  Nucleic Acids Res       Date:  2000-11-01       Impact factor: 16.971

8.  Methylation by a mutant T2 DNA [N(6)-adenine] methyltransferase expands the usage of RecA-assisted endonuclease (RARE) cleavage.

Authors:  I Minko; S Hattman; R S Lloyd; V Kossykh
Journal:  Nucleic Acids Res       Date:  2001-04-01       Impact factor: 16.971

9.  Comparative studies of the phage T2 and T4 DNA (N6-adenine)methyltransferases: amino acid changes that affect catalytic activity.

Authors:  V G Kossykh; S L Schlagman; S Hattman
Journal:  J Bacteriol       Date:  1997-05       Impact factor: 3.490

10.  The Rate and Spectrum of Spontaneous Mutations in Mycobacterium smegmatis, a Bacterium Naturally Devoid of the Postreplicative Mismatch Repair Pathway.

Authors:  Sibel Kucukyildirim; Hongan Long; Way Sung; Samuel F Miller; Thomas G Doak; Michael Lynch
Journal:  G3 (Bethesda)       Date:  2016-07-07       Impact factor: 3.154
  10 in total

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